Isolation and initial characterization of a novel type of Baeyer-Villiger monooxygenase activity from a marine microorganism.
Bottom Line: The enzyme is able to use NADH as the source of reducing power necessary to accept the atom of diatomic oxygen not incorporated into the oxyfunctionalized substrate.A particular feature of this BVMO lies in its truncated C-terminal domain, which results in a relatively small protein (357 amino acids; 38.4 kDa).Although low, abundance was detected in samples from many marine provinces, confirming the potential for biodiscovery in marine microorganisms.
Affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, UK. email@example.comShow MeSH
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Mentions: A BLASTp search within the NCBI database revealed a large number of similar sequences of which the 67 most similar sequences were selected for a subsequent phylogenetic analysis (Fig. 2). This analysis showed that a gene from S. maltophila strain K279a, annotated as a putative monooxygenase (Crossman et al., 2008), was closely related to that of isolate PML 168 and that these two ORFs together with the only other available Class B flavoprotein from a strain of Stenotrophomonas (S. maltophila R551‐3) formed a separate cluster (Fig. 2). The reliability of the branching order in the phylogenetic tree was evaluated by analysing the consensus between the branching order of this neighbour‐joining (NJ) based tree and that determined using two further algorithms for phylogenetic clustering similar to the approach presented by Mühling and colleagues (2008) – the minimum evolution (ME) and the maximum likelihood (ML) methods. The similarity of the three trees was assessed by calculating the branch score distances, using the programme treedist, part of the Phylip software package (http://evolution.gs.washington.edu/phylip.html; Felsenstein, 2004). This analysis revealed that the branching order within the ME‐ and NJ‐based trees was identical (score = 0), while that of the ML‐based tree was very similar to the ME and the NJ tree (score = 0.41), thus supporting the robustness of the phylogenetic clusters. Importantly, the cluster containing the Class B flavoprotein sequences of the three Stenotrophomonas strains was identical in all three trees.
Affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, UK. firstname.lastname@example.org