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A bile-inducible membrane protein mediates bifidobacterial bile resistance.

Ruiz L, O'Connell-Motherway M, Zomer A, de los Reyes-Gavilán CG, Margolles A, van Sinderen D - Microb Biotechnol (2012)

Bottom Line: The high level of similarity to bile efflux pumps from other bifidobacteria suggests a significant and general role for Bbr_0838 in bile tolerance.Insertional inactivation of Bbr_0838 in B. breve UCC2003 resulted in a strain, UCC2003:838(800) , which exhibited reduced survival upon cholate exposure as compared with the parent strain, a phenotype that was reversed when a functional, plasmid-encoded Bbr_0838 gene was introduced into UCC2003:838(800) .This study represents the first in-depth analysis of a bile-inducible locus in bifidobacteria, identifying a key gene relevant for bifidobacterial bile tolerance.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias, Villaviciosa, Asturias, Spain.

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Restoration of wild‐type phenotype in the complemented mutant strain. A. Growth curves in the presence of different cholate concentrations are presented for the complemented strain (UCC2003::838800‐pBC838) and for the mutant strain containing pBC1.2 as a control (UCC2003::838800‐pBC1.2). Presented data are means of three independent experiments. B. Bile survival determined by colony counts of cells exposed to 0.1% sodium cholate.
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f4: Restoration of wild‐type phenotype in the complemented mutant strain. A. Growth curves in the presence of different cholate concentrations are presented for the complemented strain (UCC2003::838800‐pBC838) and for the mutant strain containing pBC1.2 as a control (UCC2003::838800‐pBC1.2). Presented data are means of three independent experiments. B. Bile survival determined by colony counts of cells exposed to 0.1% sodium cholate.

Mentions: In order to show that the reduced bile survival phenotype in the insertion mutant was due to the absence of functional Bbr_0838, we complemented UCC2003::838800 strain with a plasmid containing the structural gene. Complementation of UCC2003::838800 with Bbr_0838 expressed from its own promoter on pBC1.2 restored the growth in the presence of cholate and the bile resistant phenotype to levels comparable with wild‐type B. breve UCC2003 (Fig. 4B).


A bile-inducible membrane protein mediates bifidobacterial bile resistance.

Ruiz L, O'Connell-Motherway M, Zomer A, de los Reyes-Gavilán CG, Margolles A, van Sinderen D - Microb Biotechnol (2012)

Restoration of wild‐type phenotype in the complemented mutant strain. A. Growth curves in the presence of different cholate concentrations are presented for the complemented strain (UCC2003::838800‐pBC838) and for the mutant strain containing pBC1.2 as a control (UCC2003::838800‐pBC1.2). Presented data are means of three independent experiments. B. Bile survival determined by colony counts of cells exposed to 0.1% sodium cholate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815329&req=5

f4: Restoration of wild‐type phenotype in the complemented mutant strain. A. Growth curves in the presence of different cholate concentrations are presented for the complemented strain (UCC2003::838800‐pBC838) and for the mutant strain containing pBC1.2 as a control (UCC2003::838800‐pBC1.2). Presented data are means of three independent experiments. B. Bile survival determined by colony counts of cells exposed to 0.1% sodium cholate.
Mentions: In order to show that the reduced bile survival phenotype in the insertion mutant was due to the absence of functional Bbr_0838, we complemented UCC2003::838800 strain with a plasmid containing the structural gene. Complementation of UCC2003::838800 with Bbr_0838 expressed from its own promoter on pBC1.2 restored the growth in the presence of cholate and the bile resistant phenotype to levels comparable with wild‐type B. breve UCC2003 (Fig. 4B).

Bottom Line: The high level of similarity to bile efflux pumps from other bifidobacteria suggests a significant and general role for Bbr_0838 in bile tolerance.Insertional inactivation of Bbr_0838 in B. breve UCC2003 resulted in a strain, UCC2003:838(800) , which exhibited reduced survival upon cholate exposure as compared with the parent strain, a phenotype that was reversed when a functional, plasmid-encoded Bbr_0838 gene was introduced into UCC2003:838(800) .This study represents the first in-depth analysis of a bile-inducible locus in bifidobacteria, identifying a key gene relevant for bifidobacterial bile tolerance.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias, Villaviciosa, Asturias, Spain.

Show MeSH
Related in: MedlinePlus