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Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces.

Fujimoto M, Yamada A, Kurosawa J, Kawata A, Beppu T, Takano H, Ueda K - Microb Biotechnol (2011)

Bottom Line: The scoC mutant of S. griseus was also defective in the extracellular activity oxidizing N,N'-dimethyl-p-phenylenediamine sulfate.Addition of 10 µM CuSO(4) repressed the activity of the conserved promoter preceding scoA and caused phenylalanine auxotrophy in some Streptomyces spp. probably because of the repression of pheA; pheA encodes prephenate dehydratase, which is located at the 3' terminus of the putative operon structure.Overall, the evidence indicates that Sco is crucial for the utilization of copper under a low-copper condition and for the activation of the multiple Cu(2+) -containing oxidases that play divergent roles in the complex physiology of Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Japan.

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Transition of pH and growth yield in a 10‐day culture of S. coelicolor A3(2) in liquid medium. The wild‐type strain (open circle) and the scoC mutant (closed triangle) were grown in Bennett's liquid medium containing 1% glucose (Glc) and maltose (Mal) without and with 10 µM CuSO4. Wet cellular weight and pH of culture broth were measured every 12 h.
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f3: Transition of pH and growth yield in a 10‐day culture of S. coelicolor A3(2) in liquid medium. The wild‐type strain (open circle) and the scoC mutant (closed triangle) were grown in Bennett's liquid medium containing 1% glucose (Glc) and maltose (Mal) without and with 10 µM CuSO4. Wet cellular weight and pH of culture broth were measured every 12 h.

Mentions: Figure 3 shows the growth profiles of the scoC mutant of S. coelicolor A3(2) cultured in Bennett's liquid medium containing glucose or maltose at 1%. Overall, the scoC mutant grew effectively; the growth yield was even higher than that of the wild type. This suggests that ScoC is involved in primary metabolism and affects some energy yielding process. A notable feature observed with respect to the scoC mutant was the remarkable pH decline during its early growth in glucose medium. The acidic pH of the culture was then neutralized and alkalified up to 8.6 (Fig. 3). A similar pH profile was also exhibited by the wild type when it was cultured in glucose medium supplemented with 400 µM of bathocuproinedisulfonic acid (BCDA), a copper‐specific chelating agent (data not shown). However, such a marked pH shift was not observed when strains were cultured in the maltose medium (Fig. 3). This raises the possibility that the ScoC‐dependent function is related to the efficiency in glucose metabolism.


Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces.

Fujimoto M, Yamada A, Kurosawa J, Kawata A, Beppu T, Takano H, Ueda K - Microb Biotechnol (2011)

Transition of pH and growth yield in a 10‐day culture of S. coelicolor A3(2) in liquid medium. The wild‐type strain (open circle) and the scoC mutant (closed triangle) were grown in Bennett's liquid medium containing 1% glucose (Glc) and maltose (Mal) without and with 10 µM CuSO4. Wet cellular weight and pH of culture broth were measured every 12 h.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815325&req=5

f3: Transition of pH and growth yield in a 10‐day culture of S. coelicolor A3(2) in liquid medium. The wild‐type strain (open circle) and the scoC mutant (closed triangle) were grown in Bennett's liquid medium containing 1% glucose (Glc) and maltose (Mal) without and with 10 µM CuSO4. Wet cellular weight and pH of culture broth were measured every 12 h.
Mentions: Figure 3 shows the growth profiles of the scoC mutant of S. coelicolor A3(2) cultured in Bennett's liquid medium containing glucose or maltose at 1%. Overall, the scoC mutant grew effectively; the growth yield was even higher than that of the wild type. This suggests that ScoC is involved in primary metabolism and affects some energy yielding process. A notable feature observed with respect to the scoC mutant was the remarkable pH decline during its early growth in glucose medium. The acidic pH of the culture was then neutralized and alkalified up to 8.6 (Fig. 3). A similar pH profile was also exhibited by the wild type when it was cultured in glucose medium supplemented with 400 µM of bathocuproinedisulfonic acid (BCDA), a copper‐specific chelating agent (data not shown). However, such a marked pH shift was not observed when strains were cultured in the maltose medium (Fig. 3). This raises the possibility that the ScoC‐dependent function is related to the efficiency in glucose metabolism.

Bottom Line: The scoC mutant of S. griseus was also defective in the extracellular activity oxidizing N,N'-dimethyl-p-phenylenediamine sulfate.Addition of 10 µM CuSO(4) repressed the activity of the conserved promoter preceding scoA and caused phenylalanine auxotrophy in some Streptomyces spp. probably because of the repression of pheA; pheA encodes prephenate dehydratase, which is located at the 3' terminus of the putative operon structure.Overall, the evidence indicates that Sco is crucial for the utilization of copper under a low-copper condition and for the activation of the multiple Cu(2+) -containing oxidases that play divergent roles in the complex physiology of Streptomyces.

View Article: PubMed Central - PubMed

Affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Japan.

Show MeSH
Related in: MedlinePlus