Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces.
Bottom Line: The scoC mutant of S. griseus was also defective in the extracellular activity oxidizing N,N'-dimethyl-p-phenylenediamine sulfate.Addition of 10 µM CuSO(4) repressed the activity of the conserved promoter preceding scoA and caused phenylalanine auxotrophy in some Streptomyces spp. probably because of the repression of pheA; pheA encodes prephenate dehydratase, which is located at the 3' terminus of the putative operon structure.Overall, the evidence indicates that Sco is crucial for the utilization of copper under a low-copper condition and for the activation of the multiple Cu(2+) -containing oxidases that play divergent roles in the complex physiology of Streptomyces.
Affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Japan.Show MeSH
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Mentions: To study the role of the Sco1/SenC family copper chaperone protein, a marker‐less knockout mutant for the corresponding coding sequence (scoC) was generated with respect to S. coelicolor A3(2) (Fig. 2). As shown in Fig. 2A (upper panels), aerial mycelium formation and pigment antibiotic production in the scoC mutant of S. coelicolor was delayed significantly. The wild type formed aerial mycelia and produced pigment antibiotics on day 2 on Bennett's medium supplied with 1% glucose, whereas the mutant was pale brown in colour and formed only vegetative hyphae. Scanning electron microscope observation (Fig. 2B) showed the presence of abundant aerial mycelia and spores in the wild type but only substrate hyphae in the scoC mutant. However, on day 5, the mutant formed aerial mycelia and pigment to the same extent as did the wild type (Fig. 2A). The delay in development of the scoC mutant was recovered by supplying 10 µM CuSO4 to the culture medium (Fig. 2A, lower panels) or by introducing an intact scoC using an integration vector (Fig. 2C). The delay of development was also observed when the mutant was cultured on the medium supplied with maltose (Fig. 2A), indicating that the phenotype is not specific to glucose. Similar delay of development was observed with respect to the scoC mutant of S. griseus (data not shown). These results indicate that the copper utilization by ScoC and subsequent activation of some copper‐dependent function(s) is crucial for the initiation of developmental growth.
Affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Japan.