Limits...
Biofilm formation in Streptococcus pneumoniae.

Domenech M, García E, Moscoso M - Microb Biotechnol (2011)

Bottom Line: Although neither the precise nature of these proteins nor the composition of the putative polysaccharide(s) is clear, it is known that choline-binding proteins are required for successful biofilm formation.However, much care needs to be taken when choosing strains for such studies because different S. pneumoniae isolates can show remarkable genomic differences.Multispecies and in vivo biofilm models must also be developed to provide a more complete understanding of biofilm formation and maintenance.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas and CIBER de Enfermedades Respiratorias, Madrid, Spain.

Show MeSH

Related in: MedlinePlus

CLSM of the biofilm formed by the S. pneumoniae R6 strain stained with calcofluor white. Bar, 25 µm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3815323&req=5

f4: CLSM of the biofilm formed by the S. pneumoniae R6 strain stained with calcofluor white. Bar, 25 µm.

Mentions: The use of calcofluor white M2R to stain non‐encapsulated pneumococcal cells has revealed that only biofilm‐growing cells (Fig. 4), but not planktonic cells (not shown), were able to bind calcofluor. This indicates that S. pneumoniae biofilms are composed of aggregates of microbial cells encased in an extracellular polysaccharide matrix (different to the CPS) that contains – at least –β‐linked d‐glycopyranosyl units (M. Domenech, M. Moscoso, E. García, in preparation), because calcofluor white M2R is a compound that binds to β‐1,3 and β‐1,4 polysaccharides (Harrington and Hageage, 2003).


Biofilm formation in Streptococcus pneumoniae.

Domenech M, García E, Moscoso M - Microb Biotechnol (2011)

CLSM of the biofilm formed by the S. pneumoniae R6 strain stained with calcofluor white. Bar, 25 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815323&req=5

f4: CLSM of the biofilm formed by the S. pneumoniae R6 strain stained with calcofluor white. Bar, 25 µm.
Mentions: The use of calcofluor white M2R to stain non‐encapsulated pneumococcal cells has revealed that only biofilm‐growing cells (Fig. 4), but not planktonic cells (not shown), were able to bind calcofluor. This indicates that S. pneumoniae biofilms are composed of aggregates of microbial cells encased in an extracellular polysaccharide matrix (different to the CPS) that contains – at least –β‐linked d‐glycopyranosyl units (M. Domenech, M. Moscoso, E. García, in preparation), because calcofluor white M2R is a compound that binds to β‐1,3 and β‐1,4 polysaccharides (Harrington and Hageage, 2003).

Bottom Line: Although neither the precise nature of these proteins nor the composition of the putative polysaccharide(s) is clear, it is known that choline-binding proteins are required for successful biofilm formation.However, much care needs to be taken when choosing strains for such studies because different S. pneumoniae isolates can show remarkable genomic differences.Multispecies and in vivo biofilm models must also be developed to provide a more complete understanding of biofilm formation and maintenance.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas and CIBER de Enfermedades Respiratorias, Madrid, Spain.

Show MeSH
Related in: MedlinePlus