Biofilm formation in Streptococcus pneumoniae.
Bottom Line: Although neither the precise nature of these proteins nor the composition of the putative polysaccharide(s) is clear, it is known that choline-binding proteins are required for successful biofilm formation.However, much care needs to be taken when choosing strains for such studies because different S. pneumoniae isolates can show remarkable genomic differences.Multispecies and in vivo biofilm models must also be developed to provide a more complete understanding of biofilm formation and maintenance.
Affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas and CIBER de Enfermedades Respiratorias, Madrid, Spain.Show MeSH
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Mentions: The use of calcofluor white M2R to stain non‐encapsulated pneumococcal cells has revealed that only biofilm‐growing cells (Fig. 4), but not planktonic cells (not shown), were able to bind calcofluor. This indicates that S. pneumoniae biofilms are composed of aggregates of microbial cells encased in an extracellular polysaccharide matrix (different to the CPS) that contains – at least –β‐linked d‐glycopyranosyl units (M. Domenech, M. Moscoso, E. García, in preparation), because calcofluor white M2R is a compound that binds to β‐1,3 and β‐1,4 polysaccharides (Harrington and Hageage, 2003).
Affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas and CIBER de Enfermedades Respiratorias, Madrid, Spain.