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Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray.

Remonsellez F, Galleguillos F, Moreno-Paz M, Parro V, Acosta M, Demergasso C - Microb Biotechnol (2009)

Bottom Line: The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques.However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones.Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.

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Community structures based on 16S rRNA genes of Bacteria and Archaea species from different strips of the industrial heap. Relative abundances of different microorganisms were evaluated by quantitative real‐time PCR of total DNA from different strips in operation until May 2008.
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f3: Community structures based on 16S rRNA genes of Bacteria and Archaea species from different strips of the industrial heap. Relative abundances of different microorganisms were evaluated by quantitative real‐time PCR of total DNA from different strips in operation until May 2008.

Mentions: The younger parts of the heap (strips) running since May 2008 (S7–S14) also showed remarkable differences in the microbial communities depending on the strip age. At. ferrooxidans phylotypes were found between 80% and 98% of abundance in the youngest strips (S13 and S14) with approximately 30 days of operation (Fig. 3). We observed one gap between the dominance of At. ferrooxidans and L. ferriphilum phylotypes in the strips S11 and S12 (150 and 120 days of operation respectively, Fig. 3), while L. ferriphilum phylotypes were found between 89% and 96% of relative abundance in the older strips (S7, S8, S9 and S10, over 250 days of operation, Fig. 3). The archaeon F. acidiphilum showed the same behaviour than L. ferriphilum but with lower abundance (4–9%). The rest of the bacteria such as At. thiooxidans, uncultured Alicyclobacillus and Sulfobacillus‐like bacteria were the less abundant (between 4.5% and 0.4%), with the exception of the strip S11 where Sulfobacillus‐like bacteria accounted for the 15% (Fig. 3). Ab. disulfidooxidans and Acidiphilium‐like bacteria species were not represented in the Fig. 3 because they showed relative abundances below 0.5%.


Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray.

Remonsellez F, Galleguillos F, Moreno-Paz M, Parro V, Acosta M, Demergasso C - Microb Biotechnol (2009)

Community structures based on 16S rRNA genes of Bacteria and Archaea species from different strips of the industrial heap. Relative abundances of different microorganisms were evaluated by quantitative real‐time PCR of total DNA from different strips in operation until May 2008.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815317&req=5

f3: Community structures based on 16S rRNA genes of Bacteria and Archaea species from different strips of the industrial heap. Relative abundances of different microorganisms were evaluated by quantitative real‐time PCR of total DNA from different strips in operation until May 2008.
Mentions: The younger parts of the heap (strips) running since May 2008 (S7–S14) also showed remarkable differences in the microbial communities depending on the strip age. At. ferrooxidans phylotypes were found between 80% and 98% of abundance in the youngest strips (S13 and S14) with approximately 30 days of operation (Fig. 3). We observed one gap between the dominance of At. ferrooxidans and L. ferriphilum phylotypes in the strips S11 and S12 (150 and 120 days of operation respectively, Fig. 3), while L. ferriphilum phylotypes were found between 89% and 96% of relative abundance in the older strips (S7, S8, S9 and S10, over 250 days of operation, Fig. 3). The archaeon F. acidiphilum showed the same behaviour than L. ferriphilum but with lower abundance (4–9%). The rest of the bacteria such as At. thiooxidans, uncultured Alicyclobacillus and Sulfobacillus‐like bacteria were the less abundant (between 4.5% and 0.4%), with the exception of the strip S11 where Sulfobacillus‐like bacteria accounted for the 15% (Fig. 3). Ab. disulfidooxidans and Acidiphilium‐like bacteria species were not represented in the Fig. 3 because they showed relative abundances below 0.5%.

Bottom Line: The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques.However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones.Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.

Show MeSH
Related in: MedlinePlus