Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray.
Bottom Line: The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques.However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones.Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM.
Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.Show MeSH
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Mentions: The younger parts of the heap (strips) running since May 2008 (S7–S14) also showed remarkable differences in the microbial communities depending on the strip age. At. ferrooxidans phylotypes were found between 80% and 98% of abundance in the youngest strips (S13 and S14) with approximately 30 days of operation (Fig. 3). We observed one gap between the dominance of At. ferrooxidans and L. ferriphilum phylotypes in the strips S11 and S12 (150 and 120 days of operation respectively, Fig. 3), while L. ferriphilum phylotypes were found between 89% and 96% of relative abundance in the older strips (S7, S8, S9 and S10, over 250 days of operation, Fig. 3). The archaeon F. acidiphilum showed the same behaviour than L. ferriphilum but with lower abundance (4–9%). The rest of the bacteria such as At. thiooxidans, uncultured Alicyclobacillus and Sulfobacillus‐like bacteria were the less abundant (between 4.5% and 0.4%), with the exception of the strip S11 where Sulfobacillus‐like bacteria accounted for the 15% (Fig. 3). Ab. disulfidooxidans and Acidiphilium‐like bacteria species were not represented in the Fig. 3 because they showed relative abundances below 0.5%.
Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.