Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time PCR and oligonucleotide prokaryotic acidophile microarray.
Bottom Line: The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques.However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones.Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM.
Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.Show MeSH
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Mentions: In order to get new insights and understanding the role that microorganisms play in mineral processing operations, we analysed the composition and the dynamic of the microbial communities in an industrial bioleaching heap in Chile (Fig. 1). To achieve this objective, we determined the active microorganisms during the leaching cycle in the industrial heap by means of a combination of two sensitive molecular techniques, such as quantitative real‐time PCR and a microarray for prokaryotic acidophiles.
Affiliation: Biotechnology Center, Universidad Católica del Norte, Avenida Angamos 0610, Antofagasta, Chile. Biotecnor Ltda., Antofagasta, Chile.