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Zn subcellular distribution in liver of goldfish (carassius auratus) with exposure to zinc oxide nanoparticles and mechanism of hepatic detoxification.

Fan W, Li Q, Yang X, Zhang L - PLoS ONE (2013)

Bottom Line: Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs.The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different.Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, School of Chemistry and Environment, Beihang University, Beijing, China.

ABSTRACT
Zinc Oxide Nanoparticles (ZnO NPs) have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut) of goldfish (Carassius auratus) after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+). And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L), bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+), the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut), rather than transport to the internal tissues (liver and muscle). The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG) increased significantly, and after Zn(2+) exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

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Zn accumulations in (A) gills, (B) gut, C) liver, and (D) muscle after 30-day exposure with ZnO NPs (nZnO), bulk ZnO (ZnO), and Zn2+.Values are means ± SD (n = 3). Bars with different letters (a, b, c) show the significant difference (p<0.05).
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pone-0078123-g003: Zn accumulations in (A) gills, (B) gut, C) liver, and (D) muscle after 30-day exposure with ZnO NPs (nZnO), bulk ZnO (ZnO), and Zn2+.Values are means ± SD (n = 3). Bars with different letters (a, b, c) show the significant difference (p<0.05).

Mentions: During the 30 day of exposure, no deaths occurred. The Zn accumulations in different tissues of the goldfish exposed to ZnO NPs, bulk ZnO, and Zn2+ after 30 days were shown in Fig. 3. Compared with the control, exposure to ZnO NPs resulted in significant Zn accumulations in the gills and liver (p<0.05), while exposure to bulk ZnO and Zn2+ both resulted in significant zinc accumulations in the gills, gut, and liver (p<0.05). Zn concentrations in different tissues were different, with the following sequence: gut>gills>liver>muscle. Overall, the tissue accumulations were similar to other literatures. Gut and gills were the crucial Zn accumulation tissues, liver came the second, and Zn contents in muscle were the least [19], [20].


Zn subcellular distribution in liver of goldfish (carassius auratus) with exposure to zinc oxide nanoparticles and mechanism of hepatic detoxification.

Fan W, Li Q, Yang X, Zhang L - PLoS ONE (2013)

Zn accumulations in (A) gills, (B) gut, C) liver, and (D) muscle after 30-day exposure with ZnO NPs (nZnO), bulk ZnO (ZnO), and Zn2+.Values are means ± SD (n = 3). Bars with different letters (a, b, c) show the significant difference (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815311&req=5

pone-0078123-g003: Zn accumulations in (A) gills, (B) gut, C) liver, and (D) muscle after 30-day exposure with ZnO NPs (nZnO), bulk ZnO (ZnO), and Zn2+.Values are means ± SD (n = 3). Bars with different letters (a, b, c) show the significant difference (p<0.05).
Mentions: During the 30 day of exposure, no deaths occurred. The Zn accumulations in different tissues of the goldfish exposed to ZnO NPs, bulk ZnO, and Zn2+ after 30 days were shown in Fig. 3. Compared with the control, exposure to ZnO NPs resulted in significant Zn accumulations in the gills and liver (p<0.05), while exposure to bulk ZnO and Zn2+ both resulted in significant zinc accumulations in the gills, gut, and liver (p<0.05). Zn concentrations in different tissues were different, with the following sequence: gut>gills>liver>muscle. Overall, the tissue accumulations were similar to other literatures. Gut and gills were the crucial Zn accumulation tissues, liver came the second, and Zn contents in muscle were the least [19], [20].

Bottom Line: Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs.The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different.Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, School of Chemistry and Environment, Beihang University, Beijing, China.

ABSTRACT
Zinc Oxide Nanoparticles (ZnO NPs) have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut) of goldfish (Carassius auratus) after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+). And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L), bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+), the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut), rather than transport to the internal tissues (liver and muscle). The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG) increased significantly, and after Zn(2+) exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

Show MeSH
Related in: MedlinePlus