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Zn subcellular distribution in liver of goldfish (carassius auratus) with exposure to zinc oxide nanoparticles and mechanism of hepatic detoxification.

Fan W, Li Q, Yang X, Zhang L - PLoS ONE (2013)

Bottom Line: Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs.The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different.Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, School of Chemistry and Environment, Beihang University, Beijing, China.

ABSTRACT
Zinc Oxide Nanoparticles (ZnO NPs) have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut) of goldfish (Carassius auratus) after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+). And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L), bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+), the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut), rather than transport to the internal tissues (liver and muscle). The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG) increased significantly, and after Zn(2+) exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

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Related in: MedlinePlus

Procedure for determining the subcellular partitioning of metal.The liver was homogenized by different treatments and centrifuged at different speeds, afterwards the following subcellular fractionations were obtained: organelles (i.e. nucleus, mitochondria, and microsomes, P2), HSP (heat-sensitive proteins, P3), MTLP (metallothionein-like protein, S3), MRG (metal-rich granules, P4), and cellular debris (S4).
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pone-0078123-g001: Procedure for determining the subcellular partitioning of metal.The liver was homogenized by different treatments and centrifuged at different speeds, afterwards the following subcellular fractionations were obtained: organelles (i.e. nucleus, mitochondria, and microsomes, P2), HSP (heat-sensitive proteins, P3), MTLP (metallothionein-like protein, S3), MRG (metal-rich granules, P4), and cellular debris (S4).

Mentions: The liver of fish collected was homogenized and centrifuged according to a procedure modified from that employed by Wallace et al. [17] (Fig. 1). Simple description was as follows:


Zn subcellular distribution in liver of goldfish (carassius auratus) with exposure to zinc oxide nanoparticles and mechanism of hepatic detoxification.

Fan W, Li Q, Yang X, Zhang L - PLoS ONE (2013)

Procedure for determining the subcellular partitioning of metal.The liver was homogenized by different treatments and centrifuged at different speeds, afterwards the following subcellular fractionations were obtained: organelles (i.e. nucleus, mitochondria, and microsomes, P2), HSP (heat-sensitive proteins, P3), MTLP (metallothionein-like protein, S3), MRG (metal-rich granules, P4), and cellular debris (S4).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815311&req=5

pone-0078123-g001: Procedure for determining the subcellular partitioning of metal.The liver was homogenized by different treatments and centrifuged at different speeds, afterwards the following subcellular fractionations were obtained: organelles (i.e. nucleus, mitochondria, and microsomes, P2), HSP (heat-sensitive proteins, P3), MTLP (metallothionein-like protein, S3), MRG (metal-rich granules, P4), and cellular debris (S4).
Mentions: The liver of fish collected was homogenized and centrifuged according to a procedure modified from that employed by Wallace et al. [17] (Fig. 1). Simple description was as follows:

Bottom Line: Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs.The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different.Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Science and Engineering, School of Chemistry and Environment, Beihang University, Beijing, China.

ABSTRACT
Zinc Oxide Nanoparticles (ZnO NPs) have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut) of goldfish (Carassius auratus) after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+). And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L), bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+), the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut), rather than transport to the internal tissues (liver and muscle). The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG) increased significantly, and after Zn(2+) exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP) were the main target for Zn(2+), while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+) were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

Show MeSH
Related in: MedlinePlus