Limits...
A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

Show MeSH

Related in: MedlinePlus

Interaction between tomato roots and Scolecobasidium humicola.Cross section of a tomato root stained with 0.005% cotton blue in 50% acetic acid 3 weeks after inoculation. The cortex (Co) mostly consists of three cell layers. Fungal hyphae can be seen on the root surface (arrows), within epidermal (Ep) cells. Vc = vascular cylinder. Bar = 20 µm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3815298&req=5

pone-0078746-g004: Interaction between tomato roots and Scolecobasidium humicola.Cross section of a tomato root stained with 0.005% cotton blue in 50% acetic acid 3 weeks after inoculation. The cortex (Co) mostly consists of three cell layers. Fungal hyphae can be seen on the root surface (arrows), within epidermal (Ep) cells. Vc = vascular cylinder. Bar = 20 µm.

Mentions: To determine the endophytic nature of S. humicola isolate H2-2, anatomical observation of tomato roots under NaNO3 or leucine treatment was conducted. The hyphae of S. humicola colonized in epidermal cells heavily, but fungal hyphea were lightly colonized in outer cortical cells. No hyphae could be observed in the inner cortical cells or in the vascular cylinder under both treatments (Figure 4). The fungal hyphae only produced conidia and microsclerotia-like-structures on the root surface under NaNO3 treatment.


A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Interaction between tomato roots and Scolecobasidium humicola.Cross section of a tomato root stained with 0.005% cotton blue in 50% acetic acid 3 weeks after inoculation. The cortex (Co) mostly consists of three cell layers. Fungal hyphae can be seen on the root surface (arrows), within epidermal (Ep) cells. Vc = vascular cylinder. Bar = 20 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815298&req=5

pone-0078746-g004: Interaction between tomato roots and Scolecobasidium humicola.Cross section of a tomato root stained with 0.005% cotton blue in 50% acetic acid 3 weeks after inoculation. The cortex (Co) mostly consists of three cell layers. Fungal hyphae can be seen on the root surface (arrows), within epidermal (Ep) cells. Vc = vascular cylinder. Bar = 20 µm.
Mentions: To determine the endophytic nature of S. humicola isolate H2-2, anatomical observation of tomato roots under NaNO3 or leucine treatment was conducted. The hyphae of S. humicola colonized in epidermal cells heavily, but fungal hyphea were lightly colonized in outer cortical cells. No hyphae could be observed in the inner cortical cells or in the vascular cylinder under both treatments (Figure 4). The fungal hyphae only produced conidia and microsclerotia-like-structures on the root surface under NaNO3 treatment.

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

Show MeSH
Related in: MedlinePlus