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A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

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Related in: MedlinePlus

Dry weights of tomato inoculated with Scolecobasidium humicola .Dry weights of tomato seedlings grown on basal media (OMA) amended with four different nitrogen sources. white bar: control, black bar: Scolecobasidium humicola H2-2, black lines bar: S. humicola F1-3, and gray lines bar: S. humicola O-MH treatments. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (P <0.05) following Tukey’s honestly significant difference test.
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pone-0078746-g003: Dry weights of tomato inoculated with Scolecobasidium humicola .Dry weights of tomato seedlings grown on basal media (OMA) amended with four different nitrogen sources. white bar: control, black bar: Scolecobasidium humicola H2-2, black lines bar: S. humicola F1-3, and gray lines bar: S. humicola O-MH treatments. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (P <0.05) following Tukey’s honestly significant difference test.

Mentions: Uninoculated (control) plants could use NaNO3, but could not effectively use three amino acids (valin, leucine, and phenylalanine). Alternatively, plants treated with three isolates, H2-2, F1-3, and O-MH, of S. humicola were able to use all 3 amino acids (Figure 3). The treated plants with H2-2 and F1-3 isolates were able to grow well on the medium amended with NaNO3 as N source, but the treated plants with O-MH isolate showed yellowing leaves and decreased biomass by 50% on the same medium. The dry weight of treated plants with 3 isolates in Valin treatment was significantly high compared to the control. When phenylalanine was used with isolate F1-3, tomato plant biomass was significantly improved but not with 2 isolates H2-2 and O-MH. Optimum plant growth was observed when the plants were treated with H2-2 and F1-3 amended with leucine as a nitrogen source at 2% concentration.


A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Dry weights of tomato inoculated with Scolecobasidium humicola .Dry weights of tomato seedlings grown on basal media (OMA) amended with four different nitrogen sources. white bar: control, black bar: Scolecobasidium humicola H2-2, black lines bar: S. humicola F1-3, and gray lines bar: S. humicola O-MH treatments. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (P <0.05) following Tukey’s honestly significant difference test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815298&req=5

pone-0078746-g003: Dry weights of tomato inoculated with Scolecobasidium humicola .Dry weights of tomato seedlings grown on basal media (OMA) amended with four different nitrogen sources. white bar: control, black bar: Scolecobasidium humicola H2-2, black lines bar: S. humicola F1-3, and gray lines bar: S. humicola O-MH treatments. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (P <0.05) following Tukey’s honestly significant difference test.
Mentions: Uninoculated (control) plants could use NaNO3, but could not effectively use three amino acids (valin, leucine, and phenylalanine). Alternatively, plants treated with three isolates, H2-2, F1-3, and O-MH, of S. humicola were able to use all 3 amino acids (Figure 3). The treated plants with H2-2 and F1-3 isolates were able to grow well on the medium amended with NaNO3 as N source, but the treated plants with O-MH isolate showed yellowing leaves and decreased biomass by 50% on the same medium. The dry weight of treated plants with 3 isolates in Valin treatment was significantly high compared to the control. When phenylalanine was used with isolate F1-3, tomato plant biomass was significantly improved but not with 2 isolates H2-2 and O-MH. Optimum plant growth was observed when the plants were treated with H2-2 and F1-3 amended with leucine as a nitrogen source at 2% concentration.

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

Show MeSH
Related in: MedlinePlus