Limits...
A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

Show MeSH

Related in: MedlinePlus

Dry weight of Chinese cabbage inoculated with different fungal isolates.Dry weight of Chinese cabbage seedlings grown on basal media (10 g oatmeal and 18 g Bacto agar, enriched with nutrients 1 g MgSO4·7H2O, 1.5 g KH2PO4, and 1 g NaNO3) inoculated with different fungal isolates. The filled columns represent the groups of selected fungi. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (** P <0.01, * P <0.05) following Tukey’s honestly significant difference test.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3815298&req=5

pone-0078746-g001: Dry weight of Chinese cabbage inoculated with different fungal isolates.Dry weight of Chinese cabbage seedlings grown on basal media (10 g oatmeal and 18 g Bacto agar, enriched with nutrients 1 g MgSO4·7H2O, 1.5 g KH2PO4, and 1 g NaNO3) inoculated with different fungal isolates. The filled columns represent the groups of selected fungi. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (** P <0.01, * P <0.05) following Tukey’s honestly significant difference test.

Mentions: To eliminate saprotrophic and/or pathogenic fungal isolates, Chinese cabbage seedlings were inoculated with 15 randomly selected isolates showing diverse morphology from each colonial morphology group. Seven isolates were originally obtained from the forest, 5 from a conventional field and 3 from an organic field. In the results of the inoculation test, only two of the 15 isolates tested (approximately 13 %) were not pathogenic to the Chinese cabbage seedlings. These two isolates, H2-2 and F1-3, caused no visible sign of disease or decay of the seedlings. The weight of dried plants was 56 ± 11 and 59 ± 14 mg for H2-2 and F1-3, respectively, and showed no significant difference compared to the control plants (68 ± 5 mg) (Figure 1). The most ineffective isolates (over 86%), once re-inoculated in to axenically-grown Chinese cabbage seedlings, caused extremely yellowing of leaves and suppression of plant growth (Figure 1).


A new fungal endophyte, Scolecobasidium humicola, promotes tomato growth under organic nitrogen conditions.

Mahmoud RS, Narisawa K - PLoS ONE (2013)

Dry weight of Chinese cabbage inoculated with different fungal isolates.Dry weight of Chinese cabbage seedlings grown on basal media (10 g oatmeal and 18 g Bacto agar, enriched with nutrients 1 g MgSO4·7H2O, 1.5 g KH2PO4, and 1 g NaNO3) inoculated with different fungal isolates. The filled columns represent the groups of selected fungi. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (** P <0.01, * P <0.05) following Tukey’s honestly significant difference test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815298&req=5

pone-0078746-g001: Dry weight of Chinese cabbage inoculated with different fungal isolates.Dry weight of Chinese cabbage seedlings grown on basal media (10 g oatmeal and 18 g Bacto agar, enriched with nutrients 1 g MgSO4·7H2O, 1.5 g KH2PO4, and 1 g NaNO3) inoculated with different fungal isolates. The filled columns represent the groups of selected fungi. Data are the means ± SE, n = 5. Asterisks represent significant differences between each treatment and the control (** P <0.01, * P <0.05) following Tukey’s honestly significant difference test.
Mentions: To eliminate saprotrophic and/or pathogenic fungal isolates, Chinese cabbage seedlings were inoculated with 15 randomly selected isolates showing diverse morphology from each colonial morphology group. Seven isolates were originally obtained from the forest, 5 from a conventional field and 3 from an organic field. In the results of the inoculation test, only two of the 15 isolates tested (approximately 13 %) were not pathogenic to the Chinese cabbage seedlings. These two isolates, H2-2 and F1-3, caused no visible sign of disease or decay of the seedlings. The weight of dried plants was 56 ± 11 and 59 ± 14 mg for H2-2 and F1-3, respectively, and showed no significant difference compared to the control plants (68 ± 5 mg) (Figure 1). The most ineffective isolates (over 86%), once re-inoculated in to axenically-grown Chinese cabbage seedlings, caused extremely yellowing of leaves and suppression of plant growth (Figure 1).

Bottom Line: This fungus was found to grow endophylically in the roots of tomato seedlings.Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source.This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo, Japan.

ABSTRACT
A new fungal endophyte, Scolecobasidium humicola, was identified as a common dark septate endophytic fungal (DSE) species under both natural and agricultural conditions. This fungus was found to grow endophylically in the roots of tomato seedlings. Light microscopy of cross-sections of colonized tomato roots showed that the intercellular, pigmented hyphae of the fungus were mostly limited to the epidermal layer and formed outer mantle-like structures. Two isolates of S. humicola, H2-2 and F1-3, have shown the ability to increase plant biomass with an organic nitrogen source. This finding is the first report of S. humicola as an endophyte and could help to improve plant growth with organic nitrogen sources.

Show MeSH
Related in: MedlinePlus