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Environmental Microbiology meets Microbial Biotechnology.

Ramos JL, Fernández M, Solano J, Pizarro-Tobias P, Daniels C - Microb Biotechnol (2008)

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An excellent example of Environmental Microbiology meeting Microbial Biotechnology comes from the publications by ) and )... In relation to this issue, ) explore the thermodynamic aspects limiting hydrogen yield in microbial fermentations; key reactions NAD(P)H + H ↔ NAD(P) + H2 hypothetically achieve equilibrium at very low partial hydrogen pressure, and the authors probe this through the design and thermodynamic analysis of a synthetic NAD(P)H:H2 pathway in Escherichia coli BL21 (DE3)... The system consists of a ferredoxin‐dependent hydrogenase, ferredoxin as electron acceptor/donor intermediate, and a NAD(P)H:ferredoxin oxidoreductase... Results revealed that in both cases these pathways are influenced by partial headspace hydrogen pressure under closed bath conditions and that the NADPH:H2 system allows higher hydrogen accumulation than the NADH:H2 pathway... In this issue ) systematically examined the twin‐arginine translocation system for secretion of heterologous proteins... The system is superior in yield and specific activity for secreted proteins than the ‘classical’ SEC pathway... Medical and pharmaceutical industries are searching new cell targets for drug discovery... Glutamate racemase, a member of the cofactor‐independent, two thiol‐based family of amino acid racemases, has been implicated in maintaining sufficient d‐glutamate pool levels required for peptidoglycan cross‐linking; which in turn is of critical importance for bacterial growth. ) reviews the history of this enzyme, the recent biochemical and structural characterization of several isoenzymes and how a set of new inhibitors have been found... Biosensors have also been the subject of mini‐review in Microbial Biotechnology, and a model for internal calibration of biosensors was recently published... In the current issue a type of cell‐based biosensor is reported that permits one to distinguish pathogenic from non‐pathogenic strains of Bacillus cereus, a technology that holds promise for detection of pathogenic bacteria in food... The whole‐cell sensor system consists of erytrophore cells of Betta splendeus... The extension of the system to detect other pathogens is of major interest and of great importance in food biosafety.

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Environmental Microbiology meets Microbial Biotechnology.

Ramos JL, Fernández M, Solano J, Pizarro-Tobias P, Daniels C - Microb Biotechnol (2008)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815286&req=5

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

An excellent example of Environmental Microbiology meeting Microbial Biotechnology comes from the publications by ) and )... In relation to this issue, ) explore the thermodynamic aspects limiting hydrogen yield in microbial fermentations; key reactions NAD(P)H + H ↔ NAD(P) + H2 hypothetically achieve equilibrium at very low partial hydrogen pressure, and the authors probe this through the design and thermodynamic analysis of a synthetic NAD(P)H:H2 pathway in Escherichia coli BL21 (DE3)... The system consists of a ferredoxin‐dependent hydrogenase, ferredoxin as electron acceptor/donor intermediate, and a NAD(P)H:ferredoxin oxidoreductase... Results revealed that in both cases these pathways are influenced by partial headspace hydrogen pressure under closed bath conditions and that the NADPH:H2 system allows higher hydrogen accumulation than the NADH:H2 pathway... In this issue ) systematically examined the twin‐arginine translocation system for secretion of heterologous proteins... The system is superior in yield and specific activity for secreted proteins than the ‘classical’ SEC pathway... Medical and pharmaceutical industries are searching new cell targets for drug discovery... Glutamate racemase, a member of the cofactor‐independent, two thiol‐based family of amino acid racemases, has been implicated in maintaining sufficient d‐glutamate pool levels required for peptidoglycan cross‐linking; which in turn is of critical importance for bacterial growth. ) reviews the history of this enzyme, the recent biochemical and structural characterization of several isoenzymes and how a set of new inhibitors have been found... Biosensors have also been the subject of mini‐review in Microbial Biotechnology, and a model for internal calibration of biosensors was recently published... In the current issue a type of cell‐based biosensor is reported that permits one to distinguish pathogenic from non‐pathogenic strains of Bacillus cereus, a technology that holds promise for detection of pathogenic bacteria in food... The whole‐cell sensor system consists of erytrophore cells of Betta splendeus... The extension of the system to detect other pathogens is of major interest and of great importance in food biosafety.

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