Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.
Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.Show MeSH
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Mentions: Upon antibiotic supplementation (10 µg ml−1 of each antibiotic) to the continuous model (day 28 and 29) (Fig. 4), the amount of L. mucosae decreased below detection limit (= 2 log cfu ml−1) in both the M‐ and L‐SHIME. In contrast, P. acidilactici was much less affected by the antibiotic treatment and even increased after the antibiotic treatment (P = 0.037 for both M‐ and L‐SHIME). One week after the antibiotic pulse, the Lactobacilli communities in both units returned to their initial composition. The presence of a mucosal compartment allowed a faster and more complete recovery after the antibiotic pulse. Interestingly, a 3 week stabilization period allowed L. mucosae to dominate over P. acidilactici within the M‐SHIME, while the inverse was true for the L‐SHIME. The level of L. mucosae was significantly higher in the M‐SHIME compared with the L‐SHIME (P = 0.009).
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.