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Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.

Van den Abbeele P, Roos S, Eeckhaut V, MacKenzie DA, Derde M, Verstraete W, Marzorati M, Possemiers S, Vanhoecke B, Van Immerseel F, Van de Wiele T - Microb Biotechnol (2011)

Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

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Abundance of Pediococcus acidilactici and Lactobacillus mucosae (log cfu ml−1) as determined with plate counts on a Lactobacillus‐specific growth medium (LAMVAB) in the luminal content of the M‐SHIME (A) and L‐SHIME (B). Results are represented in function of the time after inoculation (days). An antibiotic pulse with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin was applied on two consecutive days (day 28 and 29 after inoculation). The detection limit of the plate count‐method was 2 log cfu ml−1.
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f4: Abundance of Pediococcus acidilactici and Lactobacillus mucosae (log cfu ml−1) as determined with plate counts on a Lactobacillus‐specific growth medium (LAMVAB) in the luminal content of the M‐SHIME (A) and L‐SHIME (B). Results are represented in function of the time after inoculation (days). An antibiotic pulse with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin was applied on two consecutive days (day 28 and 29 after inoculation). The detection limit of the plate count‐method was 2 log cfu ml−1.

Mentions: Upon antibiotic supplementation (10 µg ml−1 of each antibiotic) to the continuous model (day 28 and 29) (Fig. 4), the amount of L. mucosae decreased below detection limit (= 2 log cfu ml−1) in both the M‐ and L‐SHIME. In contrast, P. acidilactici was much less affected by the antibiotic treatment and even increased after the antibiotic treatment (P = 0.037 for both M‐ and L‐SHIME). One week after the antibiotic pulse, the Lactobacilli communities in both units returned to their initial composition. The presence of a mucosal compartment allowed a faster and more complete recovery after the antibiotic pulse. Interestingly, a 3 week stabilization period allowed L. mucosae to dominate over P. acidilactici within the M‐SHIME, while the inverse was true for the L‐SHIME. The level of L. mucosae was significantly higher in the M‐SHIME compared with the L‐SHIME (P = 0.009).


Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.

Van den Abbeele P, Roos S, Eeckhaut V, MacKenzie DA, Derde M, Verstraete W, Marzorati M, Possemiers S, Vanhoecke B, Van Immerseel F, Van de Wiele T - Microb Biotechnol (2011)

Abundance of Pediococcus acidilactici and Lactobacillus mucosae (log cfu ml−1) as determined with plate counts on a Lactobacillus‐specific growth medium (LAMVAB) in the luminal content of the M‐SHIME (A) and L‐SHIME (B). Results are represented in function of the time after inoculation (days). An antibiotic pulse with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin was applied on two consecutive days (day 28 and 29 after inoculation). The detection limit of the plate count‐method was 2 log cfu ml−1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815277&req=5

f4: Abundance of Pediococcus acidilactici and Lactobacillus mucosae (log cfu ml−1) as determined with plate counts on a Lactobacillus‐specific growth medium (LAMVAB) in the luminal content of the M‐SHIME (A) and L‐SHIME (B). Results are represented in function of the time after inoculation (days). An antibiotic pulse with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin was applied on two consecutive days (day 28 and 29 after inoculation). The detection limit of the plate count‐method was 2 log cfu ml−1.
Mentions: Upon antibiotic supplementation (10 µg ml−1 of each antibiotic) to the continuous model (day 28 and 29) (Fig. 4), the amount of L. mucosae decreased below detection limit (= 2 log cfu ml−1) in both the M‐ and L‐SHIME. In contrast, P. acidilactici was much less affected by the antibiotic treatment and even increased after the antibiotic treatment (P = 0.037 for both M‐ and L‐SHIME). One week after the antibiotic pulse, the Lactobacilli communities in both units returned to their initial composition. The presence of a mucosal compartment allowed a faster and more complete recovery after the antibiotic pulse. Interestingly, a 3 week stabilization period allowed L. mucosae to dominate over P. acidilactici within the M‐SHIME, while the inverse was true for the L‐SHIME. The level of L. mucosae was significantly higher in the M‐SHIME compared with the L‐SHIME (P = 0.009).

Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

Show MeSH
Related in: MedlinePlus