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Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.

Van den Abbeele P, Roos S, Eeckhaut V, MacKenzie DA, Derde M, Verstraete W, Marzorati M, Possemiers S, Vanhoecke B, Van Immerseel F, Van de Wiele T - Microb Biotechnol (2011)

Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

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Clustering tree based on Pearson and UPGMA correlation of total bacterial (A) Lactobacillus‐specific (B/C/D) DGGE profiles of samples from the M‐SHIME (lumen and mucus) and L‐SHIME (lumen) on day 1 (A/B). On day 3, both the M‐ and L‐SHIME were inoculated with LGG and samples were taken on day 4, 6 and 8 (C). Finally, a long‐term stabilized SHIME was treated with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin on two consecutive days (day 28 and 29) and samples were analysed on day 28 (before treatment), 31 and 39 (D). The bands corresponding to pure cultures of Pediococcus acidilactici (1), Lactobacillus mucosae (2) and Lactobacillus rhamnosus GG (LGG; 3) are indicated with rectangle 1, 2 and 3 respectively.
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f2: Clustering tree based on Pearson and UPGMA correlation of total bacterial (A) Lactobacillus‐specific (B/C/D) DGGE profiles of samples from the M‐SHIME (lumen and mucus) and L‐SHIME (lumen) on day 1 (A/B). On day 3, both the M‐ and L‐SHIME were inoculated with LGG and samples were taken on day 4, 6 and 8 (C). Finally, a long‐term stabilized SHIME was treated with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin on two consecutive days (day 28 and 29) and samples were analysed on day 28 (before treatment), 31 and 39 (D). The bands corresponding to pure cultures of Pediococcus acidilactici (1), Lactobacillus mucosae (2) and Lactobacillus rhamnosus GG (LGG; 3) are indicated with rectangle 1, 2 and 3 respectively.

Mentions: One day after inoculation, DGGE analysis for the total bacterial community revealed distinct differences between the microbiota of the luminal and the mucosal environment (< 60% similarity between mucus and lumen) (Fig. 2A). Interestingly, both the M‐ and L‐SHIME contained fairly similar microbial communities in their luminal environment (≈ 90% similarity).


Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.

Van den Abbeele P, Roos S, Eeckhaut V, MacKenzie DA, Derde M, Verstraete W, Marzorati M, Possemiers S, Vanhoecke B, Van Immerseel F, Van de Wiele T - Microb Biotechnol (2011)

Clustering tree based on Pearson and UPGMA correlation of total bacterial (A) Lactobacillus‐specific (B/C/D) DGGE profiles of samples from the M‐SHIME (lumen and mucus) and L‐SHIME (lumen) on day 1 (A/B). On day 3, both the M‐ and L‐SHIME were inoculated with LGG and samples were taken on day 4, 6 and 8 (C). Finally, a long‐term stabilized SHIME was treated with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin on two consecutive days (day 28 and 29) and samples were analysed on day 28 (before treatment), 31 and 39 (D). The bands corresponding to pure cultures of Pediococcus acidilactici (1), Lactobacillus mucosae (2) and Lactobacillus rhamnosus GG (LGG; 3) are indicated with rectangle 1, 2 and 3 respectively.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3815277&req=5

f2: Clustering tree based on Pearson and UPGMA correlation of total bacterial (A) Lactobacillus‐specific (B/C/D) DGGE profiles of samples from the M‐SHIME (lumen and mucus) and L‐SHIME (lumen) on day 1 (A/B). On day 3, both the M‐ and L‐SHIME were inoculated with LGG and samples were taken on day 4, 6 and 8 (C). Finally, a long‐term stabilized SHIME was treated with 10 µg l−1 tetracycline, amoxicillin and ciprofloxacin on two consecutive days (day 28 and 29) and samples were analysed on day 28 (before treatment), 31 and 39 (D). The bands corresponding to pure cultures of Pediococcus acidilactici (1), Lactobacillus mucosae (2) and Lactobacillus rhamnosus GG (LGG; 3) are indicated with rectangle 1, 2 and 3 respectively.
Mentions: One day after inoculation, DGGE analysis for the total bacterial community revealed distinct differences between the microbiota of the luminal and the mucosal environment (< 60% similarity between mucus and lumen) (Fig. 2A). Interestingly, both the M‐ and L‐SHIME contained fairly similar microbial communities in their luminal environment (≈ 90% similarity).

Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

Show MeSH
Related in: MedlinePlus