Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.
Bottom Line: Short-term assays confirmed the strong mucin-binding of both L. mucosae and LGG compared with P.acidilactici.The mucosal environment also increased long-term colonization of L. mucosae and enhanced its stability upon antibiotic treatment (tetracycline, amoxicillin and ciprofloxacin).Incorporating a mucosal environment thus allowed colonization of specific microbes such as L. mucosae and LGG, in correspondence with the in vivo situation.
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.Show MeSH
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Mentions: One day after inoculation, DGGE analysis for the total bacterial community revealed distinct differences between the microbiota of the luminal and the mucosal environment (< 60% similarity between mucus and lumen) (Fig. 2A). Interestingly, both the M‐ and L‐SHIME contained fairly similar microbial communities in their luminal environment (≈ 90% similarity).
Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.