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Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture.

Asok A, Arshad E, Jasmin C, Pai SS, Singh IS, Mohandas A, Anas A - Microb Biotechnol (2011)

Bottom Line: We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems.This resulted in the death of >50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization.In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.

View Article: PubMed Central - PubMed

Affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi 682016, India.

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A. Death rate of V. harveyi at increasing concentrations of photosensitizer and exposure time. Values expressed as average ± SE. No significant differences between the death rates at various concentrations (P >0.05). B. Growth of V. harveyi after photoexcitation in the presence of 30 µM RB for an incubation period of 0, 10, 20 and 30 min respectively (representative plates).
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f1: A. Death rate of V. harveyi at increasing concentrations of photosensitizer and exposure time. Values expressed as average ± SE. No significant differences between the death rates at various concentrations (P >0.05). B. Growth of V. harveyi after photoexcitation in the presence of 30 µM RB for an incubation period of 0, 10, 20 and 30 min respectively (representative plates).

Mentions: We observed a time‐dependent increase in the death rate of V. harveyi at increasing concentration of RB (Fig. 1A). Interestingly, > 50% death occurred within the first 10 min of photo excitation at 20–50 µM concentrations RB, peaking to 80–100% over 30 min. Figure 1B shows the representative images of the responses of V. harveyi to photoexcitation in the presence of 30 µM RB. Complete elimination of V. harveyi was achieved at 30 µM concentration of RB after 30 min photoexcitation. Less than 20% death of V. harveyi was observed at the highest concentration (50 µM) of RB alone (Fig. S1) or when exposed to light without photosensitizer.


Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture.

Asok A, Arshad E, Jasmin C, Pai SS, Singh IS, Mohandas A, Anas A - Microb Biotechnol (2011)

A. Death rate of V. harveyi at increasing concentrations of photosensitizer and exposure time. Values expressed as average ± SE. No significant differences between the death rates at various concentrations (P >0.05). B. Growth of V. harveyi after photoexcitation in the presence of 30 µM RB for an incubation period of 0, 10, 20 and 30 min respectively (representative plates).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815272&req=5

f1: A. Death rate of V. harveyi at increasing concentrations of photosensitizer and exposure time. Values expressed as average ± SE. No significant differences between the death rates at various concentrations (P >0.05). B. Growth of V. harveyi after photoexcitation in the presence of 30 µM RB for an incubation period of 0, 10, 20 and 30 min respectively (representative plates).
Mentions: We observed a time‐dependent increase in the death rate of V. harveyi at increasing concentration of RB (Fig. 1A). Interestingly, > 50% death occurred within the first 10 min of photo excitation at 20–50 µM concentrations RB, peaking to 80–100% over 30 min. Figure 1B shows the representative images of the responses of V. harveyi to photoexcitation in the presence of 30 µM RB. Complete elimination of V. harveyi was achieved at 30 µM concentration of RB after 30 min photoexcitation. Less than 20% death of V. harveyi was observed at the highest concentration (50 µM) of RB alone (Fig. S1) or when exposed to light without photosensitizer.

Bottom Line: We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems.This resulted in the death of >50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization.In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.

View Article: PubMed Central - PubMed

Affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi 682016, India.

Show MeSH
Related in: MedlinePlus