Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture.
Bottom Line: We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems.This resulted in the death of >50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization.In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.
Affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi 682016, India.Show MeSH
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Mentions: We observed a time‐dependent increase in the death rate of V. harveyi at increasing concentration of RB (Fig. 1A). Interestingly, > 50% death occurred within the first 10 min of photo excitation at 20–50 µM concentrations RB, peaking to 80–100% over 30 min. Figure 1B shows the representative images of the responses of V. harveyi to photoexcitation in the presence of 30 µM RB. Complete elimination of V. harveyi was achieved at 30 µM concentration of RB after 30 min photoexcitation. Less than 20% death of V. harveyi was observed at the highest concentration (50 µM) of RB alone (Fig. S1) or when exposed to light without photosensitizer.
Affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi 682016, India.