The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.
Bottom Line: Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected.Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids.Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees.
Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.Show MeSH
Related in: MedlinePlus
Mentions: We revisited the glycerophospholipid inventory of organic solvent‐sensitive and solvent‐tolerant P. putida strains, namely the GRAS classified strain P. putida KT2440, the solvent‐tolerant strains P. putida DOT‐T1E and S12, and the multipurpose [e.g. biocatalysis and solvent tolerance (Park et al., 2007), biofilm producing (Gross et al., 2007)] strain Pseudomonas sp. VLB120. Distinct species of phosphatidylethanolamine, phosphatidylglycerol and cardiolipin were characterized referring to the number of carbon atoms and unsaturated carbon bonds (Fig. 3A), with more detailed information given in Table S1. The novel LIT‐FTICR‐MS technique was thereby complemented with GC/MS analysis of the hydrolysed glycerophospholipids to obtain the relative amounts of the total fatty acid moieties (Fig. 3B).
Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.