The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.
Bottom Line: Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected.Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids.Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees.
Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.Show MeSH
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Mentions: Cell membranes consist of a multiplicity of individual protein and lipid species with the main constituents belonging to only few distinct glycerophospholipid classes. The cytoplasmic membrane of many bacteria, including proteobacteria, as well as the inner side of the outer membrane mainly contains phosphatidylethanolamine (PE), phosphatidylglycerol (PG), cardiolipin (CL) and the respective monoacyl‐glycerophospholipids (lyso‐PE, lyso‐PG) (Fig. 1). The latter are part of the de‐acylation/re‐acylation cycle to control the overall lipid species compositions, catalysed by phospholipases, such as phospholipase A2 and lyso‐phospholipases that specifically release fatty acids from the sn2 position of the glycerol backbone (Scheer et al., 2011).
Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.