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The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.

Rühl J, Hein EM, Hayen H, Schmid A, Blank LM - Microb Biotechnol (2011)

Bottom Line: Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected.Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids.Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.

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Glycerophospholipid classes of P. putida. PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin. lyso‐glycerophospholipid species are representative examples with the acyl moiety in sn1 position. Alkyl1–4 represents alkyl moieties (same or different ones).
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f1: Glycerophospholipid classes of P. putida. PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin. lyso‐glycerophospholipid species are representative examples with the acyl moiety in sn1 position. Alkyl1–4 represents alkyl moieties (same or different ones).

Mentions: Cell membranes consist of a multiplicity of individual protein and lipid species with the main constituents belonging to only few distinct glycerophospholipid classes. The cytoplasmic membrane of many bacteria, including proteobacteria, as well as the inner side of the outer membrane mainly contains phosphatidylethanolamine (PE), phosphatidylglycerol (PG), cardiolipin (CL) and the respective monoacyl‐glycerophospholipids (lyso‐PE, lyso‐PG) (Fig. 1). The latter are part of the de‐acylation/re‐acylation cycle to control the overall lipid species compositions, catalysed by phospholipases, such as phospholipase A2 and lyso‐phospholipases that specifically release fatty acids from the sn2 position of the glycerol backbone (Scheer et al., 2011).


The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.

Rühl J, Hein EM, Hayen H, Schmid A, Blank LM - Microb Biotechnol (2011)

Glycerophospholipid classes of P. putida. PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin. lyso‐glycerophospholipid species are representative examples with the acyl moiety in sn1 position. Alkyl1–4 represents alkyl moieties (same or different ones).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815271&req=5

f1: Glycerophospholipid classes of P. putida. PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin. lyso‐glycerophospholipid species are representative examples with the acyl moiety in sn1 position. Alkyl1–4 represents alkyl moieties (same or different ones).
Mentions: Cell membranes consist of a multiplicity of individual protein and lipid species with the main constituents belonging to only few distinct glycerophospholipid classes. The cytoplasmic membrane of many bacteria, including proteobacteria, as well as the inner side of the outer membrane mainly contains phosphatidylethanolamine (PE), phosphatidylglycerol (PG), cardiolipin (CL) and the respective monoacyl‐glycerophospholipids (lyso‐PE, lyso‐PG) (Fig. 1). The latter are part of the de‐acylation/re‐acylation cycle to control the overall lipid species compositions, catalysed by phospholipases, such as phospholipase A2 and lyso‐phospholipases that specifically release fatty acids from the sn2 position of the glycerol backbone (Scheer et al., 2011).

Bottom Line: Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected.Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids.Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.

Show MeSH
Related in: MedlinePlus