Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440.
Bottom Line: The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production.Our results demonstrate that the intracellular presence of PHA granules confers resistance to cell envelope.Conditions to control the cell autolysis in P. putida BXHL in terms of optimal fermentation, PHA content and PHA recovery have been set up by exploring the sensitivity to detergents, chelating agents and wet biomass solubility in organic solvents such as ethyl acetate.
Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, Madrid, Spain.Show MeSH
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Mentions: Our previous results indicated that cells expressing the ejh‐ejl cassette were more sensitive to outer membrane disturbing agents, such as chelating agents and detergents, when growing in rich medium than under optimal PHA production conditions in nitrogen limited minimal medium. Therefore, to improve the cell disruption for recovering PHA granules we tested a different strategy to alter the stability of the cell envelope. With this aim, we used tol‐pal mutants of P. putida KT2440 as recipients of the lytic system instead of wild‐type strain, because these mutants exhibit severe alterations in outer membrane integrity (Table 1). A comprehensive scheme of the Tol/Pal membrane proteins is depicted in Fig. 8.
Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, Madrid, Spain.