Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440.
Bottom Line: The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production.Our results demonstrate that the intracellular presence of PHA granules confers resistance to cell envelope.Conditions to control the cell autolysis in P. putida BXHL in terms of optimal fermentation, PHA content and PHA recovery have been set up by exploring the sensitivity to detergents, chelating agents and wet biomass solubility in organic solvents such as ethyl acetate.
Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, Madrid, Spain.Show MeSH
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Mentions: As an alternative culture approach, we established a two steps culture fermentation system where cells are initially cultured in a rich medium, and afterwards transferred to the PHA production medium (see Experimental procedures). Table 3 shows the growth parameters concerning P. putida KT2440 and KTHL strains cultured in a two steps system. When 3MB was added to the culture medium at the beginning of the second step, the viability of the KTHL cells was significantly reduced (Fig. 3) but biomass and PHA yield reached similar levels to those obtained with the wild‐type strain or with non‐induced KTHL strain (Table 3). Therefore, the two steps fermentation strategy was selected as the optimal method for producing PHA in P. putida KTHL strain.
Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, Madrid, Spain.