Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.
Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.
Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.Show MeSH
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Mentions: For the detection of one or more compounds responsible for the antifungal activity, and therefore probably for biocontrol, the crude methanolic extracts from the dried and acid precipitated supernatant fluid of B. subtilis HC8 were profiled on thin layer chromatography (TLC) plates, using iturin A as a reference antibiotic (Fig. 3A). We have also profiled Bacillus amyloliquefaciens FZB42 to evaluate the similarity/difference between two beneficial strains.
Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.