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Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.

Malfanova N, Kamilova F, Validov S, Shcherbakov A, Chebotar V, Tikhonovich I, Lugtenberg B - Microb Biotechnol (2011)

Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.

View Article: PubMed Central - PubMed

Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.

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Related in: MedlinePlus

Evaluation of antifungal metabolites produced by B. subtilis HC8 and B. amyloliquefaciens FZB42. A. TLC analysis of methanol extract of the supernatant fluids of Bacillus strains. The plate was developed in chloroform/methanol/water 65:25:4 (v/v/v) for 2.5 h. For visualization, the developed plate was stained in iodine followed by dipping in 1% aqueous starch. Pure iturin A (It) was used as a reference. HC8, endophytic strain B. subtilis HC8; FZB42, Bacillus amyloliquefaciens FZB42; ΔbmyA, mutant of FZB42 unable to produce bacillomycin D; ΔfenA, mutant of FZB42 unable to produce fengycin; t‐z, major spots of the HC8 crude extract; z, likely correspond to iturin; s, fraction likely to contain bacillomycin D; w and t likely to contain fengycin. B. Antifungal activity of individual fractions of crude extract from B. subtilis HC8 towards Forl in vitro. t‐z, major fractions corresponding to spots in A).
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f3: Evaluation of antifungal metabolites produced by B. subtilis HC8 and B. amyloliquefaciens FZB42. A. TLC analysis of methanol extract of the supernatant fluids of Bacillus strains. The plate was developed in chloroform/methanol/water 65:25:4 (v/v/v) for 2.5 h. For visualization, the developed plate was stained in iodine followed by dipping in 1% aqueous starch. Pure iturin A (It) was used as a reference. HC8, endophytic strain B. subtilis HC8; FZB42, Bacillus amyloliquefaciens FZB42; ΔbmyA, mutant of FZB42 unable to produce bacillomycin D; ΔfenA, mutant of FZB42 unable to produce fengycin; t‐z, major spots of the HC8 crude extract; z, likely correspond to iturin; s, fraction likely to contain bacillomycin D; w and t likely to contain fengycin. B. Antifungal activity of individual fractions of crude extract from B. subtilis HC8 towards Forl in vitro. t‐z, major fractions corresponding to spots in A).

Mentions: For the detection of one or more compounds responsible for the antifungal activity, and therefore probably for biocontrol, the crude methanolic extracts from the dried and acid precipitated supernatant fluid of B. subtilis HC8 were profiled on thin layer chromatography (TLC) plates, using iturin A as a reference antibiotic (Fig. 3A). We have also profiled Bacillus amyloliquefaciens FZB42 to evaluate the similarity/difference between two beneficial strains.


Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.

Malfanova N, Kamilova F, Validov S, Shcherbakov A, Chebotar V, Tikhonovich I, Lugtenberg B - Microb Biotechnol (2011)

Evaluation of antifungal metabolites produced by B. subtilis HC8 and B. amyloliquefaciens FZB42. A. TLC analysis of methanol extract of the supernatant fluids of Bacillus strains. The plate was developed in chloroform/methanol/water 65:25:4 (v/v/v) for 2.5 h. For visualization, the developed plate was stained in iodine followed by dipping in 1% aqueous starch. Pure iturin A (It) was used as a reference. HC8, endophytic strain B. subtilis HC8; FZB42, Bacillus amyloliquefaciens FZB42; ΔbmyA, mutant of FZB42 unable to produce bacillomycin D; ΔfenA, mutant of FZB42 unable to produce fengycin; t‐z, major spots of the HC8 crude extract; z, likely correspond to iturin; s, fraction likely to contain bacillomycin D; w and t likely to contain fengycin. B. Antifungal activity of individual fractions of crude extract from B. subtilis HC8 towards Forl in vitro. t‐z, major fractions corresponding to spots in A).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3815264&req=5

f3: Evaluation of antifungal metabolites produced by B. subtilis HC8 and B. amyloliquefaciens FZB42. A. TLC analysis of methanol extract of the supernatant fluids of Bacillus strains. The plate was developed in chloroform/methanol/water 65:25:4 (v/v/v) for 2.5 h. For visualization, the developed plate was stained in iodine followed by dipping in 1% aqueous starch. Pure iturin A (It) was used as a reference. HC8, endophytic strain B. subtilis HC8; FZB42, Bacillus amyloliquefaciens FZB42; ΔbmyA, mutant of FZB42 unable to produce bacillomycin D; ΔfenA, mutant of FZB42 unable to produce fengycin; t‐z, major spots of the HC8 crude extract; z, likely correspond to iturin; s, fraction likely to contain bacillomycin D; w and t likely to contain fengycin. B. Antifungal activity of individual fractions of crude extract from B. subtilis HC8 towards Forl in vitro. t‐z, major fractions corresponding to spots in A).
Mentions: For the detection of one or more compounds responsible for the antifungal activity, and therefore probably for biocontrol, the crude methanolic extracts from the dried and acid precipitated supernatant fluid of B. subtilis HC8 were profiled on thin layer chromatography (TLC) plates, using iturin A as a reference antibiotic (Fig. 3A). We have also profiled Bacillus amyloliquefaciens FZB42 to evaluate the similarity/difference between two beneficial strains.

Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.

View Article: PubMed Central - PubMed

Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus