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Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.

Malfanova N, Kamilova F, Validov S, Shcherbakov A, Chebotar V, Tikhonovich I, Lugtenberg B - Microb Biotechnol (2011)

Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.

View Article: PubMed Central - PubMed

Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.

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Related in: MedlinePlus

Biocontrol of TFRR in stone wool substrate by endophytic bacteria. Tomato seeds were inoculated with a suspension of bacterial cells except for the control (C) and grown in stone wool plugs with added spores. Each variant consisted of 4 replicas with 30 plants each. Numbers inside the columns present the percentage of sick plants scored 2 weeks after inoculation. Bars indicate confidence interval (P < 0.05). Statistically different values are indicated with asterisks. (A) and (B) represent different experiments.
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f2: Biocontrol of TFRR in stone wool substrate by endophytic bacteria. Tomato seeds were inoculated with a suspension of bacterial cells except for the control (C) and grown in stone wool plugs with added spores. Each variant consisted of 4 replicas with 30 plants each. Numbers inside the columns present the percentage of sick plants scored 2 weeks after inoculation. Bars indicate confidence interval (P < 0.05). Statistically different values are indicated with asterisks. (A) and (B) represent different experiments.

Mentions: The three B. subtilis strains, BT18, HC8 and MZ3, were selected as the best antagonists (Table 2). Therefore, their ability to control TFRR was evaluated. Seed bacterization with only HC8 significantly decreased disease symptoms, from 91% to 42% (Fig. 2A). Significant biocontrol activity of HC8 was also found in a second experiment (Fig. 2B). Although the two other antagonistic strains, MZ3 and BT18, did not show significant biocontrol of TFRR (Fig. 2A) plants bacterized with these strains did show reduced disease severity (results not shown).


Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.

Malfanova N, Kamilova F, Validov S, Shcherbakov A, Chebotar V, Tikhonovich I, Lugtenberg B - Microb Biotechnol (2011)

Biocontrol of TFRR in stone wool substrate by endophytic bacteria. Tomato seeds were inoculated with a suspension of bacterial cells except for the control (C) and grown in stone wool plugs with added spores. Each variant consisted of 4 replicas with 30 plants each. Numbers inside the columns present the percentage of sick plants scored 2 weeks after inoculation. Bars indicate confidence interval (P < 0.05). Statistically different values are indicated with asterisks. (A) and (B) represent different experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815264&req=5

f2: Biocontrol of TFRR in stone wool substrate by endophytic bacteria. Tomato seeds were inoculated with a suspension of bacterial cells except for the control (C) and grown in stone wool plugs with added spores. Each variant consisted of 4 replicas with 30 plants each. Numbers inside the columns present the percentage of sick plants scored 2 weeks after inoculation. Bars indicate confidence interval (P < 0.05). Statistically different values are indicated with asterisks. (A) and (B) represent different experiments.
Mentions: The three B. subtilis strains, BT18, HC8 and MZ3, were selected as the best antagonists (Table 2). Therefore, their ability to control TFRR was evaluated. Seed bacterization with only HC8 significantly decreased disease symptoms, from 91% to 42% (Fig. 2A). Significant biocontrol activity of HC8 was also found in a second experiment (Fig. 2B). Although the two other antagonistic strains, MZ3 and BT18, did not show significant biocontrol of TFRR (Fig. 2A) plants bacterized with these strains did show reduced disease severity (results not shown).

Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.

View Article: PubMed Central - PubMed

Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.

Show MeSH
Related in: MedlinePlus