Characterization of Bacillus subtilis HC8, a novel plant-beneficial endophytic strain from giant hogweed.
Bottom Line: Metabolites possibly responsible for these plant-beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively.However, thin layer chromatography profiles of the two strains differ.It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.
Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.Show MeSH
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Mentions: Four endophytic strains, namely BT18, HC2, HC8 and MZ3, were tested for their ability to promote the growth of radish plants in non‐sterile potting soil (Fig. 1). Radish was chosen as the model plant because its roots secrete a high level of tryptophan (Kamilova et al., 2006), which can be used by many beneficial bacteria as the precursor of auxin. The only tested strain that was able to increase the root weight of radish plants was B. subtilis HC8 (Fig. 1). The root weight was chosen because this is the commercially interesting plant part. Strain HC8 significantly enhanced fresh root biomass, with as much as 46% compared with uninoculated control plants. Inoculation with the auxin‐producing strain R. aquatilis HC2 and with B. subtilis BT18 did not show a significant increase of root growth. B. subtilis strain MZ3 decreased the root biomass, but not significantly.
Affiliation: Leiden University, Institute of Biology, Sylvius Laboratory, Sylviusweg 72, Leiden, The Netherlands.