Whole-cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila.
Bottom Line: In the present paper, we report results with two gene constructs using the Tetrahymena thermophila MTT1 and MTT5 metallothionein promoters linked with the eukaryotic luciferase gene as a reporter.Validation of these whole-cell biosensors was carried out using both artificial and natural samples, including methods for detecting false positives and negatives.Comparison with other published cell biosensors indicates that the Tetrahymena metallothionein promoter-based biosensors appear to be the most sensitive eukaryotic metal biosensors and compare favourably with some prokaryotic biosensors as well.
Affiliation: Departamento de Microbiología-III, Facultad de Biología, C/. José Antonio Novais 2, Universidad Complutense, Madrid, Spain.Show MeSH
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Mentions: Both WCBs (MTT1Luc and MTT5Luc) were likewise exposed to environmental samples whose metal pollution had been determined by conventional analytic methodology, i.e, the methods specified by recent legislation. Consistent with its high sensitivity, the MTT5Luc strain reported the presence of metals in all samples, with the strongest bioluminescence induced by the most metal‐polluted sample (#4) (Fig. 4). The MTT1Luc strain showed induced bioluminescence in response to a subset of the samples (# 4, 7 and 8) (Fig. 4).
Affiliation: Departamento de Microbiología-III, Facultad de Biología, C/. José Antonio Novais 2, Universidad Complutense, Madrid, Spain.