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Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureus.

Wu XC, Qian CD, Fang HH, Wen YP, Zhou JY, Zhan ZJ, Ding R, Li O, Gao H - Microb Biotechnol (2010)

Bottom Line: Elucidation of the structure by nuclear magnetic resonance and infrared spectroscopy revealed that the active compound, paenimacrolidin (PAM), was a novel 22-membered macrolide with side-chains.The antibiotic capacity of PAM was compromised by its instability, which can be overcome significantly with addition of an anti-oxidant.To our knowledge, this is the first report of the isolation of an active macrolide from paenibacilli, which may be a promising source of novel antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China. mblab@163.com

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Related in: MedlinePlus

The relationship of the antibiotic activity of PAM and its stability. MIC was defined as the lowest antibiotic concentration that completely prevented visible growth of MRSA ATCC43300 after incubation at 37°C for 18 h. A. PAM (128 µg ml−1) was prepared in sterile Mueller–Hinton broth without () or with VC (208 µg ml−1) (▴), and incubated at 37°C. Compared with the initial concentration of PAM, the ratio of the remaining PAM was determined after incubation at 37°C for 6, 12, 18 and 24 h, by HPLC analysis on a Diamonsil C18 reversed‐phase column. B. The MICs of PAM or VAN without or with VC in the molar ratio of 1:25 and 1:5, respectively. Abbreviations: PAM, paenimacrolidin; VAN, vancomycin; VC, l‐ascorbic acid.
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f5: The relationship of the antibiotic activity of PAM and its stability. MIC was defined as the lowest antibiotic concentration that completely prevented visible growth of MRSA ATCC43300 after incubation at 37°C for 18 h. A. PAM (128 µg ml−1) was prepared in sterile Mueller–Hinton broth without () or with VC (208 µg ml−1) (▴), and incubated at 37°C. Compared with the initial concentration of PAM, the ratio of the remaining PAM was determined after incubation at 37°C for 6, 12, 18 and 24 h, by HPLC analysis on a Diamonsil C18 reversed‐phase column. B. The MICs of PAM or VAN without or with VC in the molar ratio of 1:25 and 1:5, respectively. Abbreviations: PAM, paenimacrolidin; VAN, vancomycin; VC, l‐ascorbic acid.

Mentions: Considering that PAM is a member of the polyene family, which is sensitive to pH, temperature, oxygen and light, we speculate that the unusual high MICs of PAM may be due to its instability. To this end, the stability of PAM in Mueller–Hinton broth at 37°C was accessed. As shown in Fig. 5A, the remaining PAM was rapidly reduced to 20.6%, 6%, 3.8%, 0% of the initial concentration (128 µg ml−1) at 6, 12, 18 and 24 h after inoculation, respectively. A similar phenomenon was observed in LB medium at 37°C or 28°C (data not shown). These data indicate that the decay of PAM appears to be exponential with a half‐life of approximately 4 h, suggesting that the chemical is unstable.


Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureus.

Wu XC, Qian CD, Fang HH, Wen YP, Zhou JY, Zhan ZJ, Ding R, Li O, Gao H - Microb Biotechnol (2010)

The relationship of the antibiotic activity of PAM and its stability. MIC was defined as the lowest antibiotic concentration that completely prevented visible growth of MRSA ATCC43300 after incubation at 37°C for 18 h. A. PAM (128 µg ml−1) was prepared in sterile Mueller–Hinton broth without () or with VC (208 µg ml−1) (▴), and incubated at 37°C. Compared with the initial concentration of PAM, the ratio of the remaining PAM was determined after incubation at 37°C for 6, 12, 18 and 24 h, by HPLC analysis on a Diamonsil C18 reversed‐phase column. B. The MICs of PAM or VAN without or with VC in the molar ratio of 1:25 and 1:5, respectively. Abbreviations: PAM, paenimacrolidin; VAN, vancomycin; VC, l‐ascorbic acid.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815261&req=5

f5: The relationship of the antibiotic activity of PAM and its stability. MIC was defined as the lowest antibiotic concentration that completely prevented visible growth of MRSA ATCC43300 after incubation at 37°C for 18 h. A. PAM (128 µg ml−1) was prepared in sterile Mueller–Hinton broth without () or with VC (208 µg ml−1) (▴), and incubated at 37°C. Compared with the initial concentration of PAM, the ratio of the remaining PAM was determined after incubation at 37°C for 6, 12, 18 and 24 h, by HPLC analysis on a Diamonsil C18 reversed‐phase column. B. The MICs of PAM or VAN without or with VC in the molar ratio of 1:25 and 1:5, respectively. Abbreviations: PAM, paenimacrolidin; VAN, vancomycin; VC, l‐ascorbic acid.
Mentions: Considering that PAM is a member of the polyene family, which is sensitive to pH, temperature, oxygen and light, we speculate that the unusual high MICs of PAM may be due to its instability. To this end, the stability of PAM in Mueller–Hinton broth at 37°C was accessed. As shown in Fig. 5A, the remaining PAM was rapidly reduced to 20.6%, 6%, 3.8%, 0% of the initial concentration (128 µg ml−1) at 6, 12, 18 and 24 h after inoculation, respectively. A similar phenomenon was observed in LB medium at 37°C or 28°C (data not shown). These data indicate that the decay of PAM appears to be exponential with a half‐life of approximately 4 h, suggesting that the chemical is unstable.

Bottom Line: Elucidation of the structure by nuclear magnetic resonance and infrared spectroscopy revealed that the active compound, paenimacrolidin (PAM), was a novel 22-membered macrolide with side-chains.The antibiotic capacity of PAM was compromised by its instability, which can be overcome significantly with addition of an anti-oxidant.To our knowledge, this is the first report of the isolation of an active macrolide from paenibacilli, which may be a promising source of novel antibiotics.

View Article: PubMed Central - PubMed

Affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China. mblab@163.com

Show MeSH
Related in: MedlinePlus