Paenimacrolidin, a novel macrolide antibiotic from Paenibacillus sp. F6-B70 active against methicillin-resistant Staphylococcus aureus.
Bottom Line: Elucidation of the structure by nuclear magnetic resonance and infrared spectroscopy revealed that the active compound, paenimacrolidin (PAM), was a novel 22-membered macrolide with side-chains.The antibiotic capacity of PAM was compromised by its instability, which can be overcome significantly with addition of an anti-oxidant.To our knowledge, this is the first report of the isolation of an active macrolide from paenibacilli, which may be a promising source of novel antibiotics.
Affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China. email@example.comShow MeSH
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Mentions: Using a competitive inhibition method, a large number of soil bacterial isolates were screened for antimicrobial activity against MRSA ATCC43300 and several dozens showed a distinct area of inhibition on nutrient agar. To rapidly select new strains with a high potential to produce novel antibiotics, 16S rDNA sequences of these obtained isolates were amplified, sequenced and analysed. The 16S rDNA (GenBank Accession No. GQ240305) of one isolate, namely F6‐B70, exhibited a low similarity to that of known bacteria. A consensus phylogenetic tree was constructed (Fig. 1) based on the almost‐complete 16S rDNA gene sequences of F6‐B70 and closely related type strains. F6‐B70 formed a distinct branch within the genus Paenibacillus and was closely related to Paenibacillus ehimensis DSM 11029T (Kim et al., 2004; Lee et al., 2004), with a sequence similarity of 97.1%. The DNA–DNA re‐association between F6‐B70 and P. ehimensis DSM 11029T was 21.8%. All these data indicate that F6‐B70 is a new member of the genus Paenibacillus.
Affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, China. firstname.lastname@example.org