Extraction, purification and identification of bacterial signal molecules based on N-acyl homoserine lactones.
Bottom Line: There are many different kinds of signal molecules in the bacterial community.In this article, different methods, including extraction, purification and characterization of HSLs, are reviewed.The review provides an insight into identification and characterization of new HSLs and other signal molecules for bacterial intercellular communication.
Affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China.Show MeSH
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Mentions: To elucidate the structure of HSLs, HSLs have to be isolated and purified from other molecules. Scientists have successfully developed a variety of analytical methods to characterize HSLs as well. Recently, two papers (Fekete et al., 2007; Kumari et al., 2008) have reviewed some methods used in their experiments. However, none of them reviewed the methods as an actual process. In this paper, we review most of the methods that have been developed to isolate, purify and characterize HSLs one by one, and a flow chart of these methods is shown in Fig. 1.
Affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China.