Small-molecule elicitation of microbial secondary metabolites.
Bottom Line: Bacterial and fungal sequencing studies indicate that the biosynthetic potential of many strains is much greater than that observed by fermentation.A targeted approach related to cultivation-based methods is the application of small-molecule elicitors to specifically affect transcription of secondary metabolite gene clusters.With the isolation of the novel secondary metabolites lunalides A and B, oxylipins, cladochromes F and G, nygerone A, chaetoglobosin-542, -540 and -510, sphaerolone, dihydrosphaerolone, mutolide and pestalone, and the enhanced production of known secondary metabolites like penicillin and bacitracin, chemical elicitation is proving to be an effective way to augment natural product libraries.
Affiliation: Cancer Research Institute and Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-2404, USA. firstname.lastname@example.orgShow MeSH
Mentions: Potentially interesting gene clusters, which may encode metabolites that increase competitiveness in natural environments, can remain silent in the unnatural setting of the microbiology laboratory. There are now many examples of bacterial and fungal biosynthetic secondary metabolite gene clusters outnumbering the number of natural products actually synthesized in the laboratory (Gross, 2009) (Fig. 1). These silent (cryptic) gene clusters undoubtedly harbour an enormous reservoir of novel bioactive constituents for drug discovery.
Affiliation: Cancer Research Institute and Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-2404, USA. email@example.com