Erythrophore cell response to food-associated pathogenic bacteria: implications for detection.
Bottom Line: Evaluation of erythrophore cell response, using Bacillus spp., has revealed that this response can distinguish pathogenic Bacillus cereus from a non-pathogenic B. cereus ΔplcR deletion mutant and a non-pathogenic Bacillus subtilis.Erythrophore cells were exposed to Salmonella enteritidis, Clostridium perfringens and Clostridium botulinum.Each bacterial pathogen elicited a response from erythrophore cells that was distinguished from the corresponding bacterial growth medium, and this observed response was unique for each bacterial pathogen.
Affiliation: Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331-3804, USA.Show MeSH
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Mentions: Clostridium botulinum was chosen as a second representative of a Gram‐positive, spore forming, yet anaerobic bacterium associated with food contamination and potential use as a biological weapon. Erythrophore cells were challenged with C. botulinum NCTC 7272 (Type A) or C. botulinum NCTC 7273 (Type B) cultured in BHI. Erythrophore cell response was monitored for 1 h. Very little response to C. botulinum was observed during this hour; therefore monitoring time was extended to 6 h. Two hours after exposure to C. botulinum NCTC 7272 or NCTC 7273, pigment organelles began to slowly aggregate (Fig. 3A) and aggregation of pigment organelles was complete at approximately 5 h. The erythrophore response to both C. botulinum cultures was statistically significant compared with the negative control (BHI) (C. botulinum NCTC 7272 P < 0.005 and C. botulinum NCTC 7273 P < 0.006 respectively). The observed morphological changes of the erythrophore cells were unique from changes induced by other bacterial pathogens. Extensive dendrite formation was observed in erythrophore cells treated with C. botulinum (Fig. 3C), while other bacterial pathogens induced pigment organelles to centrally localize within the erythrophore cell (Fig. 1C).
Affiliation: Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331-3804, USA.