Limits...
Cryptic genetic diversity is paramount in small-bodied amphibians of the genus Euparkerella (Anura: Craugastoridae) endemic to the Brazilian Atlantic forest.

Fusinatto LA, Alexandrino J, Haddad CF, Brunes TO, Rocha CF, Sequeira F - PLoS ONE (2013)

Bottom Line: Morphological similarity associated to restricted distributions and low dispersal abilities make the direct developing "Terrarana" frogs of the genus Euparkerella a good model for examining diversification processes.The analysis of 39 individuals from the four known Euparkerella species uncovered high levels of genetic diversity, especially within the two previously morphologically-defined E. cochranae and E. brasiliensis.These six units were also uncovered in the Bayesian clustering analysis, and supported by the Bayesian coalescent-based species delimitation (BPP), and Genealogical Sorting Index (GSI), providing thus strong evidence for underestimation of the current levels of diversity within Euparkerella.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ecologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil.

ABSTRACT
Morphological similarity associated to restricted distributions and low dispersal abilities make the direct developing "Terrarana" frogs of the genus Euparkerella a good model for examining diversification processes. We here infer phylogenetic relationships within the genus Euparkerella, using DNA sequence data from one mitochondrial and four nuclear genes coupled with traditional Bayesian phylogenetic reconstruction approaches and more recent coalescent methods of species tree inference. We also used Bayesian clustering analysis and a recent Bayesian coalescent-based approach specifically to infer species delimitation. The analysis of 39 individuals from the four known Euparkerella species uncovered high levels of genetic diversity, especially within the two previously morphologically-defined E. cochranae and E. brasiliensis. Within these species, the gene trees at five independent loci and trees from combined data (concatenated dataset and the species tree) uncovered six deeply diverged and geographically coherent evolutionary units, which may have diverged between the Miocene and the Pleistocene. These six units were also uncovered in the Bayesian clustering analysis, and supported by the Bayesian coalescent-based species delimitation (BPP), and Genealogical Sorting Index (GSI), providing thus strong evidence for underestimation of the current levels of diversity within Euparkerella. The cryptic diversity now uncovered opens new opportunities to examine the origins and maintenance of microendemism in the context of spatial heterogeneity and/or human induced fragmentation of the highly threatened Brazilian Atlantic forest hotspot.

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Structure results for Euparkerella populations.Assignment proportion of individuals to populations of Euparkerella from Rio de Janeiro State. Structure results for number of groups K=6. Left bar includes both mitochondrial and nuclear genes, right bar includes only nuclear genes.
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pone-0079504-g006: Structure results for Euparkerella populations.Assignment proportion of individuals to populations of Euparkerella from Rio de Janeiro State. Structure results for number of groups K=6. Left bar includes both mitochondrial and nuclear genes, right bar includes only nuclear genes.

Mentions: The level of genetic structure recovered by the Bayesian clustering methods for both nuclear and combined nuclear and mtDNA datasets of E. brasiliensis, E. cochranae, and Euparkerella sp. was K = 6, as suggested by both the highest log posterior probability of the data (ln Pr (X/K) and the ΔK score (Figure 6). Each of the six sampled localities of Rio de Janeiro was classified into distinct genetic clusters with high individual membership coefficient (0.95 to 1.0). Moreover, there is an overall concordance between these six distinct clusters with terminal clades inferred by phylogenetic analyses.


Cryptic genetic diversity is paramount in small-bodied amphibians of the genus Euparkerella (Anura: Craugastoridae) endemic to the Brazilian Atlantic forest.

Fusinatto LA, Alexandrino J, Haddad CF, Brunes TO, Rocha CF, Sequeira F - PLoS ONE (2013)

Structure results for Euparkerella populations.Assignment proportion of individuals to populations of Euparkerella from Rio de Janeiro State. Structure results for number of groups K=6. Left bar includes both mitochondrial and nuclear genes, right bar includes only nuclear genes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815154&req=5

pone-0079504-g006: Structure results for Euparkerella populations.Assignment proportion of individuals to populations of Euparkerella from Rio de Janeiro State. Structure results for number of groups K=6. Left bar includes both mitochondrial and nuclear genes, right bar includes only nuclear genes.
Mentions: The level of genetic structure recovered by the Bayesian clustering methods for both nuclear and combined nuclear and mtDNA datasets of E. brasiliensis, E. cochranae, and Euparkerella sp. was K = 6, as suggested by both the highest log posterior probability of the data (ln Pr (X/K) and the ΔK score (Figure 6). Each of the six sampled localities of Rio de Janeiro was classified into distinct genetic clusters with high individual membership coefficient (0.95 to 1.0). Moreover, there is an overall concordance between these six distinct clusters with terminal clades inferred by phylogenetic analyses.

Bottom Line: Morphological similarity associated to restricted distributions and low dispersal abilities make the direct developing "Terrarana" frogs of the genus Euparkerella a good model for examining diversification processes.The analysis of 39 individuals from the four known Euparkerella species uncovered high levels of genetic diversity, especially within the two previously morphologically-defined E. cochranae and E. brasiliensis.These six units were also uncovered in the Bayesian clustering analysis, and supported by the Bayesian coalescent-based species delimitation (BPP), and Genealogical Sorting Index (GSI), providing thus strong evidence for underestimation of the current levels of diversity within Euparkerella.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ecologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil.

ABSTRACT
Morphological similarity associated to restricted distributions and low dispersal abilities make the direct developing "Terrarana" frogs of the genus Euparkerella a good model for examining diversification processes. We here infer phylogenetic relationships within the genus Euparkerella, using DNA sequence data from one mitochondrial and four nuclear genes coupled with traditional Bayesian phylogenetic reconstruction approaches and more recent coalescent methods of species tree inference. We also used Bayesian clustering analysis and a recent Bayesian coalescent-based approach specifically to infer species delimitation. The analysis of 39 individuals from the four known Euparkerella species uncovered high levels of genetic diversity, especially within the two previously morphologically-defined E. cochranae and E. brasiliensis. Within these species, the gene trees at five independent loci and trees from combined data (concatenated dataset and the species tree) uncovered six deeply diverged and geographically coherent evolutionary units, which may have diverged between the Miocene and the Pleistocene. These six units were also uncovered in the Bayesian clustering analysis, and supported by the Bayesian coalescent-based species delimitation (BPP), and Genealogical Sorting Index (GSI), providing thus strong evidence for underestimation of the current levels of diversity within Euparkerella. The cryptic diversity now uncovered opens new opportunities to examine the origins and maintenance of microendemism in the context of spatial heterogeneity and/or human induced fragmentation of the highly threatened Brazilian Atlantic forest hotspot.

Show MeSH
Related in: MedlinePlus