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Genetic variation of drought tolerance in Pinus pinaster at three hierarchical levels: a comparison of induced osmotic stress and field testing.

Gaspar MJ, Velasco T, Feito I, Alía R, Majada J - PLoS ONE (2013)

Bottom Line: This study demonstrates the importance of additive vs non additive effects for drought tolerance traits in Pinus pinaster, and shows differences in parameters determining the adaptive trajectories of populations and family and clones within populations.Survival in the induced osmotic stress experiment presented significant correlations with survival in the xeric site, and height growth at the mesic site, at population level, indicating constraints of adaptation for those traits, while at the within population level no significant correlation existed.These results demonstrate that population differentiation and within population genetic variation for drought tolerance follow different patterns.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética e Biotecnologia, Universidade de Trás os Montes e Alto Douro, Vila Real, Portugal ; Centro de Estudos Florestais, Instituto Superior de Agronomia, ULisboa, Tapada da Ajuda, Lisboa, Portugal.

ABSTRACT
Understanding the survival capacity of forest trees to periods of severe water stress could improve knowledge of the adaptive potential of different species under future climatic scenarios. In long lived organisms, like forest trees, the combination of induced osmotic stress treatments and field testing can elucidate the role of drought tolerance during the early stages of establishment, the most critical in the life of the species. We performed a Polyethylene glycol-osmotic induced stress experiment and evaluated two common garden experiments (xeric and mesic sites) to test for survival and growth of a wide range clonal collection of Maritime pine. This study demonstrates the importance of additive vs non additive effects for drought tolerance traits in Pinus pinaster, and shows differences in parameters determining the adaptive trajectories of populations and family and clones within populations. The results show that osmotic adjustment plays an important role in population variation, while biomass allocation and hydric content greatly influence survival at population level. Survival in the induced osmotic stress experiment presented significant correlations with survival in the xeric site, and height growth at the mesic site, at population level, indicating constraints of adaptation for those traits, while at the within population level no significant correlation existed. These results demonstrate that population differentiation and within population genetic variation for drought tolerance follow different patterns.

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Regressions between provenance BLUE’s (a, b) and family BLUP’S (c, d) of S100 with Height (b, d) and Survival (a, c) evaluated in the two field experiments (circle: Xeric Conditions, triangle: Mesic conditions).
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pone-0079094-g002: Regressions between provenance BLUE’s (a, b) and family BLUP’S (c, d) of S100 with Height (b, d) and Survival (a, c) evaluated in the two field experiments (circle: Xeric Conditions, triangle: Mesic conditions).

Mentions: Significant correlations were found between some traits (Table 2). T50 and S100 were highly correlated (0.98±0.02, α<0.001) and both T50 and S100 were significantly correlated with M_HT (α<0.001) and X_SV (α<0.05) (Figure 2). Moreover, there was a high degree of correlation between height measured in the two sites (0.73±0.154; α<0.001) which indicates a consistence in the behaviour of populations in distinct environments.


Genetic variation of drought tolerance in Pinus pinaster at three hierarchical levels: a comparison of induced osmotic stress and field testing.

Gaspar MJ, Velasco T, Feito I, Alía R, Majada J - PLoS ONE (2013)

Regressions between provenance BLUE’s (a, b) and family BLUP’S (c, d) of S100 with Height (b, d) and Survival (a, c) evaluated in the two field experiments (circle: Xeric Conditions, triangle: Mesic conditions).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815124&req=5

pone-0079094-g002: Regressions between provenance BLUE’s (a, b) and family BLUP’S (c, d) of S100 with Height (b, d) and Survival (a, c) evaluated in the two field experiments (circle: Xeric Conditions, triangle: Mesic conditions).
Mentions: Significant correlations were found between some traits (Table 2). T50 and S100 were highly correlated (0.98±0.02, α<0.001) and both T50 and S100 were significantly correlated with M_HT (α<0.001) and X_SV (α<0.05) (Figure 2). Moreover, there was a high degree of correlation between height measured in the two sites (0.73±0.154; α<0.001) which indicates a consistence in the behaviour of populations in distinct environments.

Bottom Line: This study demonstrates the importance of additive vs non additive effects for drought tolerance traits in Pinus pinaster, and shows differences in parameters determining the adaptive trajectories of populations and family and clones within populations.Survival in the induced osmotic stress experiment presented significant correlations with survival in the xeric site, and height growth at the mesic site, at population level, indicating constraints of adaptation for those traits, while at the within population level no significant correlation existed.These results demonstrate that population differentiation and within population genetic variation for drought tolerance follow different patterns.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética e Biotecnologia, Universidade de Trás os Montes e Alto Douro, Vila Real, Portugal ; Centro de Estudos Florestais, Instituto Superior de Agronomia, ULisboa, Tapada da Ajuda, Lisboa, Portugal.

ABSTRACT
Understanding the survival capacity of forest trees to periods of severe water stress could improve knowledge of the adaptive potential of different species under future climatic scenarios. In long lived organisms, like forest trees, the combination of induced osmotic stress treatments and field testing can elucidate the role of drought tolerance during the early stages of establishment, the most critical in the life of the species. We performed a Polyethylene glycol-osmotic induced stress experiment and evaluated two common garden experiments (xeric and mesic sites) to test for survival and growth of a wide range clonal collection of Maritime pine. This study demonstrates the importance of additive vs non additive effects for drought tolerance traits in Pinus pinaster, and shows differences in parameters determining the adaptive trajectories of populations and family and clones within populations. The results show that osmotic adjustment plays an important role in population variation, while biomass allocation and hydric content greatly influence survival at population level. Survival in the induced osmotic stress experiment presented significant correlations with survival in the xeric site, and height growth at the mesic site, at population level, indicating constraints of adaptation for those traits, while at the within population level no significant correlation existed. These results demonstrate that population differentiation and within population genetic variation for drought tolerance follow different patterns.

Show MeSH
Related in: MedlinePlus