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Endoglin (CD105) is a more appropriate marker than CD31 for detecting microvessels in carotid artery plaques.

Fukumitsu R, Takagi Y, Yoshida K, Miyamoto S - Surg Neurol Int (2013)

Bottom Line: The average numbers of microvessels were 5.8 ± 5.4 for CD31 and 9.2 ± 9.3 for CD105 (P = 0.04).More microvessels were positive for CD105 than there were for CD31 in patients with diabetes mellitus (P = 0.04).In patients with diabetes mellitus, CD105 is significantly more highly expressed in microvessels than CD31.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Kyoto University School of Medicine, Kyoto, Japan.

ABSTRACT

Background: Microvascular proliferation is a major risk factor for plaque vulnerability in patients with carotid stenosis. There are several vascular endothelial markers such as CD31 and CD105, but it is unclear which marker is most sensitive for microvessels. This study sought to examine the correlations between CD31 and CD105 expression in microvessels on carotid plaques and clinical manifestations.

Methods: We studied 13 lesions in 12 patients. The patients underwent carotid endarterectomy and samples were stained for CD31 and CD105. The numbers of microvessels positive for these markers within a field of view were counted.

Results: The average numbers of microvessels were 5.8 ± 5.4 for CD31 and 9.2 ± 9.3 for CD105 (P = 0.04). More microvessels were positive for CD105 than there were for CD31 in patients with diabetes mellitus (P = 0.04).

Conclusion: In patients with carotid artery stenosis, CD105 is more appropriate than CD31 for detecting microvessels in carotid plaques. In patients with diabetes mellitus, CD105 is significantly more highly expressed in microvessels than CD31.

No MeSH data available.


Related in: MedlinePlus

Histological and immunohistological findings in the carotid artery plaque from patient 1. (a) H and E stain, (b) Elastica von Gieson stain, (c) CD31 stain (×100), (d) CD105 stain (×100), (e) CD31 stain (×400). The microvascular endothelial cells are stained faintly, (f) CD105 stain (×400). The microvascular endothelial cells are strongly stained in the whole circumference. The arrows show the microvessels stained negatively for CD31 and positively for CD105
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Figure 3: Histological and immunohistological findings in the carotid artery plaque from patient 1. (a) H and E stain, (b) Elastica von Gieson stain, (c) CD31 stain (×100), (d) CD105 stain (×100), (e) CD31 stain (×400). The microvascular endothelial cells are stained faintly, (f) CD105 stain (×400). The microvascular endothelial cells are strongly stained in the whole circumference. The arrows show the microvessels stained negatively for CD31 and positively for CD105

Mentions: A 78-year-old male presented left hemiparesis and magnetic resonance imaging (MRI) demonstrated cerebral infarction in the right hemisphere. He had hypertension and diabetes mellitus, and angiography revealed 65% right carotid artery stenosis. He underwent carotid endarterectomy, and the results of pathology are shown in Figure 3. The microvessels were faintly stained with CD31, but strongly expressed CD105 in the whole circumference.


Endoglin (CD105) is a more appropriate marker than CD31 for detecting microvessels in carotid artery plaques.

Fukumitsu R, Takagi Y, Yoshida K, Miyamoto S - Surg Neurol Int (2013)

Histological and immunohistological findings in the carotid artery plaque from patient 1. (a) H and E stain, (b) Elastica von Gieson stain, (c) CD31 stain (×100), (d) CD105 stain (×100), (e) CD31 stain (×400). The microvascular endothelial cells are stained faintly, (f) CD105 stain (×400). The microvascular endothelial cells are strongly stained in the whole circumference. The arrows show the microvessels stained negatively for CD31 and positively for CD105
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815080&req=5

Figure 3: Histological and immunohistological findings in the carotid artery plaque from patient 1. (a) H and E stain, (b) Elastica von Gieson stain, (c) CD31 stain (×100), (d) CD105 stain (×100), (e) CD31 stain (×400). The microvascular endothelial cells are stained faintly, (f) CD105 stain (×400). The microvascular endothelial cells are strongly stained in the whole circumference. The arrows show the microvessels stained negatively for CD31 and positively for CD105
Mentions: A 78-year-old male presented left hemiparesis and magnetic resonance imaging (MRI) demonstrated cerebral infarction in the right hemisphere. He had hypertension and diabetes mellitus, and angiography revealed 65% right carotid artery stenosis. He underwent carotid endarterectomy, and the results of pathology are shown in Figure 3. The microvessels were faintly stained with CD31, but strongly expressed CD105 in the whole circumference.

Bottom Line: The average numbers of microvessels were 5.8 ± 5.4 for CD31 and 9.2 ± 9.3 for CD105 (P = 0.04).More microvessels were positive for CD105 than there were for CD31 in patients with diabetes mellitus (P = 0.04).In patients with diabetes mellitus, CD105 is significantly more highly expressed in microvessels than CD31.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Kyoto University School of Medicine, Kyoto, Japan.

ABSTRACT

Background: Microvascular proliferation is a major risk factor for plaque vulnerability in patients with carotid stenosis. There are several vascular endothelial markers such as CD31 and CD105, but it is unclear which marker is most sensitive for microvessels. This study sought to examine the correlations between CD31 and CD105 expression in microvessels on carotid plaques and clinical manifestations.

Methods: We studied 13 lesions in 12 patients. The patients underwent carotid endarterectomy and samples were stained for CD31 and CD105. The numbers of microvessels positive for these markers within a field of view were counted.

Results: The average numbers of microvessels were 5.8 ± 5.4 for CD31 and 9.2 ± 9.3 for CD105 (P = 0.04). More microvessels were positive for CD105 than there were for CD31 in patients with diabetes mellitus (P = 0.04).

Conclusion: In patients with carotid artery stenosis, CD105 is more appropriate than CD31 for detecting microvessels in carotid plaques. In patients with diabetes mellitus, CD105 is significantly more highly expressed in microvessels than CD31.

No MeSH data available.


Related in: MedlinePlus