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Effects of aqueous leaf extract of Asystasia gangetica on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats.

Mugabo P, Raji IA - BMC Complement Altern Med (2013)

Bottom Line: The significant (p<0.05) reductions in HR were not dose-dependent.Co-infusion of A. gangetica (200 mg/kg) with either ANG I or ANG II significantly (p<0.05) suppressed the hypertensive effect of both ANG I and ANG II respectively, and was associated with reductions in HR.The reduction in BP may be a result of actions of the ALE on the ACE, the ANG II receptors and the heart rate.

View Article: PubMed Central - HTML - PubMed

Affiliation: Discipline of Pharmacology, School of Pharmacy, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa. pmugabo@uwc.ac.za.

ABSTRACT

Background: Asystasia gangentica (A. gangetica) belongs to the family Acanthaceae. It is used to treat hypertension, rheumatism, asthma, diabetes mellitus, and as an anthelmintic in South Africa, India, Cameroun, Nigeria, and Kenya respectively. It has also been reported to inhibit the angiotensin I converting enzyme (ACE) in-vitro. Therefore, the aim of this study is to investigate the in-vivo effect of aqueous leaf extract (ALE) of A. gangetica on the blood pressure (BP) and heart rate (HR) in anaesthetized male spontaneously hypertensive rats (SHR); and to elucidate possible mechanism(s) by which it acts.

Methods: The ALE of A. gangetica (10-400 mg/kg), angiotensin I human acetate salt hydrate (ANG I, 3.1-100 μg/kg) and angiotensin II human (ANG II, 3.1-50 μg/kg) were administered intravenously. The BP and HR were measured via a pressure transducer connecting the femoral artery to a Powerlab and a computer for recording.

Results: A. gangetica significantly (p<0.05), and dose-dependently reduced the systolic, diastolic, and mean arterial BP. The significant (p<0.05) reductions in HR were not dose-dependent. Both ANG I and ANG II increased the BP dose-dependently. Co-infusion of A. gangetica (200 mg/kg) with either ANG I or ANG II significantly (p<0.05) suppressed the hypertensive effect of both ANG I and ANG II respectively, and was associated with reductions in HR.

Conclusions: A. gangetica ALE reduced BP and HR in the SHR. The reduction in BP may be a result of actions of the ALE on the ACE, the ANG II receptors and the heart rate.

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Effect of angiotensin I on BP (a) and HR (b). Values are presented as mean ±. SEM. * indicates statistical significance.
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Figure 2: Effect of angiotensin I on BP (a) and HR (b). Values are presented as mean ±. SEM. * indicates statistical significance.

Mentions: ANG I (3.1–100 μg/kg) significantly (<0.01), and dose-dependently increased the maximum BP values obtained when compared to their respective values at baseline. ANG I only produced significant (<0.05) change in HR at the 3rd and 2nd highest doses (25 and 50 μg/kg respectively, Figure 2).


Effects of aqueous leaf extract of Asystasia gangetica on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats.

Mugabo P, Raji IA - BMC Complement Altern Med (2013)

Effect of angiotensin I on BP (a) and HR (b). Values are presented as mean ±. SEM. * indicates statistical significance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3815069&req=5

Figure 2: Effect of angiotensin I on BP (a) and HR (b). Values are presented as mean ±. SEM. * indicates statistical significance.
Mentions: ANG I (3.1–100 μg/kg) significantly (<0.01), and dose-dependently increased the maximum BP values obtained when compared to their respective values at baseline. ANG I only produced significant (<0.05) change in HR at the 3rd and 2nd highest doses (25 and 50 μg/kg respectively, Figure 2).

Bottom Line: The significant (p<0.05) reductions in HR were not dose-dependent.Co-infusion of A. gangetica (200 mg/kg) with either ANG I or ANG II significantly (p<0.05) suppressed the hypertensive effect of both ANG I and ANG II respectively, and was associated with reductions in HR.The reduction in BP may be a result of actions of the ALE on the ACE, the ANG II receptors and the heart rate.

View Article: PubMed Central - HTML - PubMed

Affiliation: Discipline of Pharmacology, School of Pharmacy, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa. pmugabo@uwc.ac.za.

ABSTRACT

Background: Asystasia gangentica (A. gangetica) belongs to the family Acanthaceae. It is used to treat hypertension, rheumatism, asthma, diabetes mellitus, and as an anthelmintic in South Africa, India, Cameroun, Nigeria, and Kenya respectively. It has also been reported to inhibit the angiotensin I converting enzyme (ACE) in-vitro. Therefore, the aim of this study is to investigate the in-vivo effect of aqueous leaf extract (ALE) of A. gangetica on the blood pressure (BP) and heart rate (HR) in anaesthetized male spontaneously hypertensive rats (SHR); and to elucidate possible mechanism(s) by which it acts.

Methods: The ALE of A. gangetica (10-400 mg/kg), angiotensin I human acetate salt hydrate (ANG I, 3.1-100 μg/kg) and angiotensin II human (ANG II, 3.1-50 μg/kg) were administered intravenously. The BP and HR were measured via a pressure transducer connecting the femoral artery to a Powerlab and a computer for recording.

Results: A. gangetica significantly (p<0.05), and dose-dependently reduced the systolic, diastolic, and mean arterial BP. The significant (p<0.05) reductions in HR were not dose-dependent. Both ANG I and ANG II increased the BP dose-dependently. Co-infusion of A. gangetica (200 mg/kg) with either ANG I or ANG II significantly (p<0.05) suppressed the hypertensive effect of both ANG I and ANG II respectively, and was associated with reductions in HR.

Conclusions: A. gangetica ALE reduced BP and HR in the SHR. The reduction in BP may be a result of actions of the ALE on the ACE, the ANG II receptors and the heart rate.

Show MeSH
Related in: MedlinePlus