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Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17).

Acloque H, Bonnet-Garnier A, Mompart F, Pinton A, Yerle-Bouissou M - PLoS ONE (2013)

Bottom Line: Altering this organization may interfere with the zygote development and reduce fertility or prolificity.Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization.Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR444 Génétique Cellulaire, Castanet Tolosan, France ; Université de Toulouse, INP, ENVT, UMR444 Génétique Cellulaire, Toulouse, France.

ABSTRACT
In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity. Thus, rare studies on sperm cells from infertile patients described an altered nuclear organization that may be a cause or a consequence of their respective pathologies. Thereby, chromosomal rearrangements and aneuploidy can be studied not only for their adverse effects on production of normal/balanced gametes at meiosis but also for their possible impact on sperm nuclear architecture and the epigenetic consequences of altered chromosome positioning. We decided to compare the global architecture of sperm nuclei from boars, either with a normal chromosome composition or with a Robertsonian translocation involving chromosomes 13 and 17. We hypothesized that the fusion between these chromosomes may change their spatial organization and we examined to what extend it could also modify the global sperm nuclear architecture. Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization. But specific analysis of chromosomes 13 and 17 territories highlights an influence of chromosome 17 for the positioning of the fused chromosomes within the nucleus. We also observed a specific clustering of centromeres depending of the chromosome subtypes. Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning.

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Robertsonian translocation t(13;17) affects SSC13 localization along the medio-lateral axis.A. Comparison of SSC13 and SSC17 distribution along the medio-lateral axis in control sperm nuclei. SSC13 is preferentially located at the periphery, close to the nucleus border while SSC17 is more uniformly distributed, from the periphery to the center of the nucleus. B. Comparison of SSC13 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. SSC13 is displaced toward the center of the nucleus in presence of the t(13;17). Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test. C. Comparison of SSC17 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test.
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pone-0078005-g006: Robertsonian translocation t(13;17) affects SSC13 localization along the medio-lateral axis.A. Comparison of SSC13 and SSC17 distribution along the medio-lateral axis in control sperm nuclei. SSC13 is preferentially located at the periphery, close to the nucleus border while SSC17 is more uniformly distributed, from the periphery to the center of the nucleus. B. Comparison of SSC13 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. SSC13 is displaced toward the center of the nucleus in presence of the t(13;17). Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test. C. Comparison of SSC17 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test.

Mentions: To go deeper in the analysis, we measured the distance of the center of each CT to the border of the nucleus. As expected, a significant difference between the repartition of each CT is observed (Fig. 6A). SSC13 is mostly localized at the periphery of the nucleus with less than 20% of the analyzed nuclei containing a SSC13 CT in the central part of the nucleus. SSC17 exhibits a more uniform distribution along the medio-lateral axis of the nucleus (Fig. 6A) and this repartition is also true in lymphocytes (Fig. S1).


Sperm nuclear architecture is locally modified in presence of a Robertsonian translocation t(13;17).

Acloque H, Bonnet-Garnier A, Mompart F, Pinton A, Yerle-Bouissou M - PLoS ONE (2013)

Robertsonian translocation t(13;17) affects SSC13 localization along the medio-lateral axis.A. Comparison of SSC13 and SSC17 distribution along the medio-lateral axis in control sperm nuclei. SSC13 is preferentially located at the periphery, close to the nucleus border while SSC17 is more uniformly distributed, from the periphery to the center of the nucleus. B. Comparison of SSC13 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. SSC13 is displaced toward the center of the nucleus in presence of the t(13;17). Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test. C. Comparison of SSC17 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3815027&req=5

pone-0078005-g006: Robertsonian translocation t(13;17) affects SSC13 localization along the medio-lateral axis.A. Comparison of SSC13 and SSC17 distribution along the medio-lateral axis in control sperm nuclei. SSC13 is preferentially located at the periphery, close to the nucleus border while SSC17 is more uniformly distributed, from the periphery to the center of the nucleus. B. Comparison of SSC13 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. SSC13 is displaced toward the center of the nucleus in presence of the t(13;17). Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test. C. Comparison of SSC17 distribution along the medio-lateral axis in sperm nuclei from control (gold yellow) and t(13;17) (dark green) animals. Mean values for each condition are represented above the violin plot with the p-value of the corresponding t-test.
Mentions: To go deeper in the analysis, we measured the distance of the center of each CT to the border of the nucleus. As expected, a significant difference between the repartition of each CT is observed (Fig. 6A). SSC13 is mostly localized at the periphery of the nucleus with less than 20% of the analyzed nuclei containing a SSC13 CT in the central part of the nucleus. SSC17 exhibits a more uniform distribution along the medio-lateral axis of the nucleus (Fig. 6A) and this repartition is also true in lymphocytes (Fig. S1).

Bottom Line: Altering this organization may interfere with the zygote development and reduce fertility or prolificity.Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization.Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR444 Génétique Cellulaire, Castanet Tolosan, France ; Université de Toulouse, INP, ENVT, UMR444 Génétique Cellulaire, Toulouse, France.

ABSTRACT
In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity. Thus, rare studies on sperm cells from infertile patients described an altered nuclear organization that may be a cause or a consequence of their respective pathologies. Thereby, chromosomal rearrangements and aneuploidy can be studied not only for their adverse effects on production of normal/balanced gametes at meiosis but also for their possible impact on sperm nuclear architecture and the epigenetic consequences of altered chromosome positioning. We decided to compare the global architecture of sperm nuclei from boars, either with a normal chromosome composition or with a Robertsonian translocation involving chromosomes 13 and 17. We hypothesized that the fusion between these chromosomes may change their spatial organization and we examined to what extend it could also modify the global sperm nuclear architecture. Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization. But specific analysis of chromosomes 13 and 17 territories highlights an influence of chromosome 17 for the positioning of the fused chromosomes within the nucleus. We also observed a specific clustering of centromeres depending of the chromosome subtypes. Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning.

Show MeSH
Related in: MedlinePlus