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LARP1 predict the prognosis for early-stage and AFP-normal hepatocellular carcinoma.

Xie C, Huang L, Xie S, Xie D, Zhang G, Wang P, Peng L, Gao Z - J Transl Med (2013)

Bottom Line: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression.Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# TianHe Road, Guangzhou 510630, Guangdong Province, China. happyxiechan@gmail.com.

ABSTRACT

Background: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).

Methods: LARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.

Results: mRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P<0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.

Conclusions: LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

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Related in: MedlinePlus

Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC clinical subgroups. (A) When patients were divided into subclinical groups according to tumor size, probabilities of survival with either HCC lesion diameter > 3 cm (left) or ≤3 cm (right), as well as high- and low-LARP1 expression, distinguishes lower and higher, respectively, SO rates. (B) OS in patients with single tumor lesion and multi-tumor lesion. (C) OS rates in patients subgrouped into TNM stages I-II (left) and TNM stages III (right) as differentiated by high- or low-LARP1 expression.
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Figure 4: Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC clinical subgroups. (A) When patients were divided into subclinical groups according to tumor size, probabilities of survival with either HCC lesion diameter > 3 cm (left) or ≤3 cm (right), as well as high- and low-LARP1 expression, distinguishes lower and higher, respectively, SO rates. (B) OS in patients with single tumor lesion and multi-tumor lesion. (C) OS rates in patients subgrouped into TNM stages I-II (left) and TNM stages III (right) as differentiated by high- or low-LARP1 expression.

Mentions: In another group of HCC patients whose survival has been difficult to predict in the clinic, namely, those with tumor sizes smaller than 3 cm in diameter, the 5-year survival rate was 75% in the low LARP1 group, as opposed to 23% for patients exhibiting high LARP1 expression (P = 0.005, Figure 4A). In the clinical subgroup with one single HCC tumor nodule, the 5-year survival rates were 63% and 9%, respectively, for low - or high -LARP1 expression patients (P < 0.01, Figure 4B). In early-stage HCC patients (TNM stages I-II), low -LARP1 expression patients revealed a 5-year survival rate of 53%, whereas the survival rate decreased to 13% in the high LARP1 group (P < 0.01, Figure 4C). Taken together, therefore, our data suggest a potentially promising prognostic value of LARP1 for HCC patients in various clinical subgroups that otherwise could have been difficult for survival prediction.


LARP1 predict the prognosis for early-stage and AFP-normal hepatocellular carcinoma.

Xie C, Huang L, Xie S, Xie D, Zhang G, Wang P, Peng L, Gao Z - J Transl Med (2013)

Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC clinical subgroups. (A) When patients were divided into subclinical groups according to tumor size, probabilities of survival with either HCC lesion diameter > 3 cm (left) or ≤3 cm (right), as well as high- and low-LARP1 expression, distinguishes lower and higher, respectively, SO rates. (B) OS in patients with single tumor lesion and multi-tumor lesion. (C) OS rates in patients subgrouped into TNM stages I-II (left) and TNM stages III (right) as differentiated by high- or low-LARP1 expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814951&req=5

Figure 4: Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC clinical subgroups. (A) When patients were divided into subclinical groups according to tumor size, probabilities of survival with either HCC lesion diameter > 3 cm (left) or ≤3 cm (right), as well as high- and low-LARP1 expression, distinguishes lower and higher, respectively, SO rates. (B) OS in patients with single tumor lesion and multi-tumor lesion. (C) OS rates in patients subgrouped into TNM stages I-II (left) and TNM stages III (right) as differentiated by high- or low-LARP1 expression.
Mentions: In another group of HCC patients whose survival has been difficult to predict in the clinic, namely, those with tumor sizes smaller than 3 cm in diameter, the 5-year survival rate was 75% in the low LARP1 group, as opposed to 23% for patients exhibiting high LARP1 expression (P = 0.005, Figure 4A). In the clinical subgroup with one single HCC tumor nodule, the 5-year survival rates were 63% and 9%, respectively, for low - or high -LARP1 expression patients (P < 0.01, Figure 4B). In early-stage HCC patients (TNM stages I-II), low -LARP1 expression patients revealed a 5-year survival rate of 53%, whereas the survival rate decreased to 13% in the high LARP1 group (P < 0.01, Figure 4C). Taken together, therefore, our data suggest a potentially promising prognostic value of LARP1 for HCC patients in various clinical subgroups that otherwise could have been difficult for survival prediction.

Bottom Line: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression.Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# TianHe Road, Guangzhou 510630, Guangdong Province, China. happyxiechan@gmail.com.

ABSTRACT

Background: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).

Methods: LARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.

Results: mRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P<0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.

Conclusions: LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

Show MeSH
Related in: MedlinePlus