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LARP1 predict the prognosis for early-stage and AFP-normal hepatocellular carcinoma.

Xie C, Huang L, Xie S, Xie D, Zhang G, Wang P, Peng L, Gao Z - J Transl Med (2013)

Bottom Line: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression.Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# TianHe Road, Guangzhou 510630, Guangdong Province, China. happyxiechan@gmail.com.

ABSTRACT

Background: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).

Methods: LARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.

Results: mRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P<0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.

Conclusions: LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

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Related in: MedlinePlus

Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC subgroups of different AFP levels. (A) Using AFP level (400 ng/ml) as the cut-off could not separate patients with different OS rates in the study cohort (left). By contrast, LARP1 expression level predicted different OS rate in the subgroup of AFP < 400 ng/ml and AFP ≥ 400 ng/ml. (B) OS rate of patients with AFP <200 ng/ml and AFP ≥ 200 ng/ml, respectively. (C) OS rates of patients with high- or low- LARP1 expression when further divided into AFP < 100 ng/ml and AFP ≥ 100 ng/ml subgroups.
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Figure 3: Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC subgroups of different AFP levels. (A) Using AFP level (400 ng/ml) as the cut-off could not separate patients with different OS rates in the study cohort (left). By contrast, LARP1 expression level predicted different OS rate in the subgroup of AFP < 400 ng/ml and AFP ≥ 400 ng/ml. (B) OS rate of patients with AFP <200 ng/ml and AFP ≥ 200 ng/ml, respectively. (C) OS rates of patients with high- or low- LARP1 expression when further divided into AFP < 100 ng/ml and AFP ≥ 100 ng/ml subgroups.

Mentions: A validation cohort was employed to further evaluate the prognostic value of LARP1 for specific subgroups of patients. As shown in Figure 3, the LARP1 levels were significantly associated with OS in patients with early-stage HCC, and such a predictive power was also observed in patients without AFP elevation. In the subgroup of patients with low AFP (< 400 ng/ml), LARP1-low expression was associated with a 5-year OS rate of 54%, in contrast to 13% for the high-LARP1 group (P < 0.01, Figure 3A). Then we used different cutoff levels of AFP (400 ng/ml, 200 ng/ml and 100 ng/ml, respectively) to subgroup the 272 HCC patients and evaluated the prognostic significance of LARP1in the patient subgroups.Our data suggested that AFP cut-off values of 200 ng/ml or 100 ng/ml) were significantly predictive of patient survival, whereas AFP = 400 ng/ml was not a prognostic cut-off. However, in all above HCC patient subgroups, the level of LARP1 was more sensitive to predict the prognosis of HCC patients than AFP. As shown in the subgroup of AFP level less than 200 or 100 ng/ml, LARP1 was able to separate patients with different OS rates as well (Figure 3B and C).


LARP1 predict the prognosis for early-stage and AFP-normal hepatocellular carcinoma.

Xie C, Huang L, Xie S, Xie D, Zhang G, Wang P, Peng L, Gao Z - J Transl Med (2013)

Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC subgroups of different AFP levels. (A) Using AFP level (400 ng/ml) as the cut-off could not separate patients with different OS rates in the study cohort (left). By contrast, LARP1 expression level predicted different OS rate in the subgroup of AFP < 400 ng/ml and AFP ≥ 400 ng/ml. (B) OS rate of patients with AFP <200 ng/ml and AFP ≥ 200 ng/ml, respectively. (C) OS rates of patients with high- or low- LARP1 expression when further divided into AFP < 100 ng/ml and AFP ≥ 100 ng/ml subgroups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814951&req=5

Figure 3: Kaplan-Meier analysis of OS in 272 patients based on LARP1 expressions in HCC subgroups of different AFP levels. (A) Using AFP level (400 ng/ml) as the cut-off could not separate patients with different OS rates in the study cohort (left). By contrast, LARP1 expression level predicted different OS rate in the subgroup of AFP < 400 ng/ml and AFP ≥ 400 ng/ml. (B) OS rate of patients with AFP <200 ng/ml and AFP ≥ 200 ng/ml, respectively. (C) OS rates of patients with high- or low- LARP1 expression when further divided into AFP < 100 ng/ml and AFP ≥ 100 ng/ml subgroups.
Mentions: A validation cohort was employed to further evaluate the prognostic value of LARP1 for specific subgroups of patients. As shown in Figure 3, the LARP1 levels were significantly associated with OS in patients with early-stage HCC, and such a predictive power was also observed in patients without AFP elevation. In the subgroup of patients with low AFP (< 400 ng/ml), LARP1-low expression was associated with a 5-year OS rate of 54%, in contrast to 13% for the high-LARP1 group (P < 0.01, Figure 3A). Then we used different cutoff levels of AFP (400 ng/ml, 200 ng/ml and 100 ng/ml, respectively) to subgroup the 272 HCC patients and evaluated the prognostic significance of LARP1in the patient subgroups.Our data suggested that AFP cut-off values of 200 ng/ml or 100 ng/ml) were significantly predictive of patient survival, whereas AFP = 400 ng/ml was not a prognostic cut-off. However, in all above HCC patient subgroups, the level of LARP1 was more sensitive to predict the prognosis of HCC patients than AFP. As shown in the subgroup of AFP level less than 200 or 100 ng/ml, LARP1 was able to separate patients with different OS rates as well (Figure 3B and C).

Bottom Line: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression.Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen University, 600# TianHe Road, Guangzhou 510630, Guangdong Province, China. happyxiechan@gmail.com.

ABSTRACT

Background: The La-related protein 1 (LARP1) has been found to be a RNA binding protein and was related to spermatogenesis, embryogenesis and cell-cycle progression. The aim of this study was to investigate the prognostic value of LARP1 in hepatocellular carcinoma (HCC).

Methods: LARP1 expression was examined in 15 HCC cell lines and 272 clinical specimens using real-time PCR, immunohistochemistry (IHC) and western blot analysis (WB). LARP1 expression was also studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Statistical analyses were applied to derive association between LARP1 expression scores and clinical characters as well as patient survival.

Results: mRNA and protein levels of LARP1 were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. LARP1 expression was correlated to survival time, vital status, tumor size and Child-Pugh score. Overall survival analysis showed HCC patients with high LARP1 expression level had lower survival rate (P<0.01). Importantly, this correlation remained significant in patients with early-stage HCC or with normal serum AFP level.

Conclusions: LARP1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.

Show MeSH
Related in: MedlinePlus