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Identification of 3 distinct HIV-1 founding strains responsible for expanding epidemic among men who have sex with men in 9 Chinese cities.

Han X, An M, Zhang M, Zhao B, Wu H, Liang S, Chen X, Zhuang M, Yan H, Fu J, Lu L, Cai W, Takebe Y, Shang H - J. Acquir. Immune Defic. Syndr. (2013)

Bottom Line: To investigate the epidemiological relationships between HIV-1 strains that are spread among the men who have sex with men (MSM) populations of 9 cities across China and to analyze the origins and divergence times of the major epidemic strains found in the MSM population.We identified 3 distinct HIV-1 lineages (clusters 1-3) responsible for the recent upsurge of the AIDS epidemic among MSM in China.These 3 HIV-1 variants are spread widely among MSM throughout China, demonstrating remarkable founding effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Key Laboratory of AIDS Immunology of Ministry of Health, The First Affiliated Hospital of China Medical University, Shenyang, China.

ABSTRACT

Objectives: To investigate the epidemiological relationships between HIV-1 strains that are spread among the men who have sex with men (MSM) populations of 9 cities across China and to analyze the origins and divergence times of the major epidemic strains found in the MSM population.

Methods: A total of 583 HIV-1-positive subjects were recruited from high-risk MSM populations in 9 cities across China between 2009 and 2011. Nucleotide sequences of 1.0-kb pro-RT regions were amplified and sequenced. Phylogenetic and Bayesian molecular clock analyses were performed.

Results: The overall distribution of HIV-1 genotypes was as follows: CRF01_AE, 62.1%; CRF07_BC, 18.2%; subtype B (United States-European), 15.9%; subtype B', 0.7%; other recombinants, 3.1%. In addition to the 2 distinct CRF01_AE clusters [cluster 1 (n = 157, 26.9%) and cluster 2 (n = 196, 33.6%)] previously reported by our group, we identified a novel CRF07_BC cluster (cluster 3) (n = 94, 16.1%) unique to China's MSM population whose strains were homologous and could be detected in all 9 cities. These 3 lineages of HIV-1 strains (clusters 1-3) accounted for 76.7% (447 of 583) of infections among MSM in China as a whole. Clusters 1, 2, and 3 were estimated to have been introduced into the MSM population in 1999, 2001, and 2001, respectively, indicating that the newly identified CRF07_BC cluster 3 is not a young lineage. However, it spread quickly in recent years.

Conclusions: We identified 3 distinct HIV-1 lineages (clusters 1-3) responsible for the recent upsurge of the AIDS epidemic among MSM in China. These 3 HIV-1 variants are spread widely among MSM throughout China, demonstrating remarkable founding effects.

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Geographic distribution of HIV-1 genotypes and 3 distinct phylogenetic clusters identified in this study. HIV-1 genotype distribution in their respective study cities are depicted on the map of China below. Symbols on the pie graphs indicate the following (also see inset): 1, CRF01_AE cluster 1; 2, CRF01_AE cluster 2; 01th, Thailand CRF01_AE; 3, CRF07_BC cluster 3; 07cn, typical CRF07_BC; B, subtype B (United States–European); B′, subtype B′ (Thailand variant of subtype B); O, other recombinant.
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Figure 2: Geographic distribution of HIV-1 genotypes and 3 distinct phylogenetic clusters identified in this study. HIV-1 genotype distribution in their respective study cities are depicted on the map of China below. Symbols on the pie graphs indicate the following (also see inset): 1, CRF01_AE cluster 1; 2, CRF01_AE cluster 2; 01th, Thailand CRF01_AE; 3, CRF07_BC cluster 3; 07cn, typical CRF07_BC; B, subtype B (United States–European); B′, subtype B′ (Thailand variant of subtype B); O, other recombinant.

Mentions: Figure 2 illustrates the HIV-1 genotype distribution among MSM in 9 Chinese cities (Table 1). The CRF01_AE genotype was most prevalent in 7 of the 9 cities (all except Chengdu and Dongguan): prevalence of CRF01_AE ranged from 25.0% in Dongguan to 81.0% in Shenyang. As shown in Figure 1A, we detected 2 distinct phylogenetic clusters of CRF01_AE (designated China's MSM clusters 1 and 2). Among the MSM subjects, 97.5% (353 of 362) of the circulating CRF01_AE strains belonged to either cluster 1 (157 of 362, 43.4%) or cluster 2 (196 of 362, 54.1%). A small fraction of the CRF01_AE strains (9 of 362, 2.5%) share a similar phylogenetic ancestry with strains found in typical Thailand CRF01_AE groups (Table 1; Fig. 1A). The prevalence of CRF01_AE cluster 1 is higher than that of cluster 2 in most cities, except Shenyang (capital of Liaoning province, northeastern China), where cluster 2 is the predominant lineage among the CRF01_AE strains (20.2% for cluster 1 vs. 77.5% for cluster 2) and among all HIV-1 strains circulating in Shenyang (165 of 263, 62.7%). CRF01_AE cluster 2 strains were detected in Beijing (24 of 163, 14.7%), Shanghai (3 of 20, 15.0%), Nanjing (2 of 18, 11.1%), and Changsha (2 of 23, 8.7%) but not in Jinan (0 of 10), Chengdu (0 of 63), Dongguan (0 of 16), or Kunming (0 of 7) (Table 1).


Identification of 3 distinct HIV-1 founding strains responsible for expanding epidemic among men who have sex with men in 9 Chinese cities.

Han X, An M, Zhang M, Zhao B, Wu H, Liang S, Chen X, Zhuang M, Yan H, Fu J, Lu L, Cai W, Takebe Y, Shang H - J. Acquir. Immune Defic. Syndr. (2013)

Geographic distribution of HIV-1 genotypes and 3 distinct phylogenetic clusters identified in this study. HIV-1 genotype distribution in their respective study cities are depicted on the map of China below. Symbols on the pie graphs indicate the following (also see inset): 1, CRF01_AE cluster 1; 2, CRF01_AE cluster 2; 01th, Thailand CRF01_AE; 3, CRF07_BC cluster 3; 07cn, typical CRF07_BC; B, subtype B (United States–European); B′, subtype B′ (Thailand variant of subtype B); O, other recombinant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814940&req=5

Figure 2: Geographic distribution of HIV-1 genotypes and 3 distinct phylogenetic clusters identified in this study. HIV-1 genotype distribution in their respective study cities are depicted on the map of China below. Symbols on the pie graphs indicate the following (also see inset): 1, CRF01_AE cluster 1; 2, CRF01_AE cluster 2; 01th, Thailand CRF01_AE; 3, CRF07_BC cluster 3; 07cn, typical CRF07_BC; B, subtype B (United States–European); B′, subtype B′ (Thailand variant of subtype B); O, other recombinant.
Mentions: Figure 2 illustrates the HIV-1 genotype distribution among MSM in 9 Chinese cities (Table 1). The CRF01_AE genotype was most prevalent in 7 of the 9 cities (all except Chengdu and Dongguan): prevalence of CRF01_AE ranged from 25.0% in Dongguan to 81.0% in Shenyang. As shown in Figure 1A, we detected 2 distinct phylogenetic clusters of CRF01_AE (designated China's MSM clusters 1 and 2). Among the MSM subjects, 97.5% (353 of 362) of the circulating CRF01_AE strains belonged to either cluster 1 (157 of 362, 43.4%) or cluster 2 (196 of 362, 54.1%). A small fraction of the CRF01_AE strains (9 of 362, 2.5%) share a similar phylogenetic ancestry with strains found in typical Thailand CRF01_AE groups (Table 1; Fig. 1A). The prevalence of CRF01_AE cluster 1 is higher than that of cluster 2 in most cities, except Shenyang (capital of Liaoning province, northeastern China), where cluster 2 is the predominant lineage among the CRF01_AE strains (20.2% for cluster 1 vs. 77.5% for cluster 2) and among all HIV-1 strains circulating in Shenyang (165 of 263, 62.7%). CRF01_AE cluster 2 strains were detected in Beijing (24 of 163, 14.7%), Shanghai (3 of 20, 15.0%), Nanjing (2 of 18, 11.1%), and Changsha (2 of 23, 8.7%) but not in Jinan (0 of 10), Chengdu (0 of 63), Dongguan (0 of 16), or Kunming (0 of 7) (Table 1).

Bottom Line: To investigate the epidemiological relationships between HIV-1 strains that are spread among the men who have sex with men (MSM) populations of 9 cities across China and to analyze the origins and divergence times of the major epidemic strains found in the MSM population.We identified 3 distinct HIV-1 lineages (clusters 1-3) responsible for the recent upsurge of the AIDS epidemic among MSM in China.These 3 HIV-1 variants are spread widely among MSM throughout China, demonstrating remarkable founding effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Key Laboratory of AIDS Immunology of Ministry of Health, The First Affiliated Hospital of China Medical University, Shenyang, China.

ABSTRACT

Objectives: To investigate the epidemiological relationships between HIV-1 strains that are spread among the men who have sex with men (MSM) populations of 9 cities across China and to analyze the origins and divergence times of the major epidemic strains found in the MSM population.

Methods: A total of 583 HIV-1-positive subjects were recruited from high-risk MSM populations in 9 cities across China between 2009 and 2011. Nucleotide sequences of 1.0-kb pro-RT regions were amplified and sequenced. Phylogenetic and Bayesian molecular clock analyses were performed.

Results: The overall distribution of HIV-1 genotypes was as follows: CRF01_AE, 62.1%; CRF07_BC, 18.2%; subtype B (United States-European), 15.9%; subtype B', 0.7%; other recombinants, 3.1%. In addition to the 2 distinct CRF01_AE clusters [cluster 1 (n = 157, 26.9%) and cluster 2 (n = 196, 33.6%)] previously reported by our group, we identified a novel CRF07_BC cluster (cluster 3) (n = 94, 16.1%) unique to China's MSM population whose strains were homologous and could be detected in all 9 cities. These 3 lineages of HIV-1 strains (clusters 1-3) accounted for 76.7% (447 of 583) of infections among MSM in China as a whole. Clusters 1, 2, and 3 were estimated to have been introduced into the MSM population in 1999, 2001, and 2001, respectively, indicating that the newly identified CRF07_BC cluster 3 is not a young lineage. However, it spread quickly in recent years.

Conclusions: We identified 3 distinct HIV-1 lineages (clusters 1-3) responsible for the recent upsurge of the AIDS epidemic among MSM in China. These 3 HIV-1 variants are spread widely among MSM throughout China, demonstrating remarkable founding effects.

Show MeSH
Related in: MedlinePlus