Limits...
Effect of storage levels of nitric oxide derivatives in blood components.

Qazi MA, Rizzatti F, Piknova B, Sibmooh N, Stroncek DF, Schechter AN - F1000Res (2012)

Bottom Line: Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air.Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

View Article: PubMed Central - PubMed

Affiliation: Molecular Medicine Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

ABSTRACT

Background: Potential deleterious effects of red blood cell (RBC) transfusions, especially from blood kept at length, have been ascribed to biochemical changes during storage, including those of nitric oxide (NO) metabolism. Study methods and design: In this study, NO metabolites, nitrite and nitrate, were quantified in RBCs and whole blood with time of storage. Whole blood (WB), leukoreduced (LR), and non-leukoreduced (NLR) components were obtained from healthy volunteer donors and stored in polyvinyl chloride bags for 42 days. Nitrite and nitrate were measured using reductive gas-phase chemiluminescence.

Results: Nitrite concentrations initially decreased rapidly from about 150nmol/L, but stabilized at about 44nmol/L in room air for up to 42 days. Nitrate concentrations remained stable during storage at about 35┬Ámol/L. Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air. Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.

Conclusion: As erythrocytes may contribute to the control of blood flow and oxygen delivery through reduction of nitrite to NO under hypoxic conditions, the present findings provide insight into possible effects of blood transfusion. These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

No MeSH data available.


Related in: MedlinePlus

Change in MetHb levels in room air (6A) and chamber (6B) samples over 42 days of storage; number of donors, n=2 (A), n=2 (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3814924&req=5

f6: Change in MetHb levels in room air (6A) and chamber (6B) samples over 42 days of storage; number of donors, n=2 (A), n=2 (B).

Mentions: A CO-oximeter was used to measure MetHb levels in all three blood components stored in both room air and chamber conditions.Figure 6 shows the gradual increase in MetHb from nearly 0.5% to just above 1% during the storage period. MetHb concentration is expressed as percentage of total hemoglobin concentration.


Effect of storage levels of nitric oxide derivatives in blood components.

Qazi MA, Rizzatti F, Piknova B, Sibmooh N, Stroncek DF, Schechter AN - F1000Res (2012)

Change in MetHb levels in room air (6A) and chamber (6B) samples over 42 days of storage; number of donors, n=2 (A), n=2 (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814924&req=5

f6: Change in MetHb levels in room air (6A) and chamber (6B) samples over 42 days of storage; number of donors, n=2 (A), n=2 (B).
Mentions: A CO-oximeter was used to measure MetHb levels in all three blood components stored in both room air and chamber conditions.Figure 6 shows the gradual increase in MetHb from nearly 0.5% to just above 1% during the storage period. MetHb concentration is expressed as percentage of total hemoglobin concentration.

Bottom Line: Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air.Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

View Article: PubMed Central - PubMed

Affiliation: Molecular Medicine Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

ABSTRACT

Background: Potential deleterious effects of red blood cell (RBC) transfusions, especially from blood kept at length, have been ascribed to biochemical changes during storage, including those of nitric oxide (NO) metabolism. Study methods and design: In this study, NO metabolites, nitrite and nitrate, were quantified in RBCs and whole blood with time of storage. Whole blood (WB), leukoreduced (LR), and non-leukoreduced (NLR) components were obtained from healthy volunteer donors and stored in polyvinyl chloride bags for 42 days. Nitrite and nitrate were measured using reductive gas-phase chemiluminescence.

Results: Nitrite concentrations initially decreased rapidly from about 150nmol/L, but stabilized at about 44nmol/L in room air for up to 42 days. Nitrate concentrations remained stable during storage at about 35┬Ámol/L. Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air. Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.

Conclusion: As erythrocytes may contribute to the control of blood flow and oxygen delivery through reduction of nitrite to NO under hypoxic conditions, the present findings provide insight into possible effects of blood transfusion. These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

No MeSH data available.


Related in: MedlinePlus