Limits...
Effect of storage levels of nitric oxide derivatives in blood components.

Qazi MA, Rizzatti F, Piknova B, Sibmooh N, Stroncek DF, Schechter AN - F1000Res (2012)

Bottom Line: Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air.Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

View Article: PubMed Central - PubMed

Affiliation: Molecular Medicine Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

ABSTRACT

Background: Potential deleterious effects of red blood cell (RBC) transfusions, especially from blood kept at length, have been ascribed to biochemical changes during storage, including those of nitric oxide (NO) metabolism. Study methods and design: In this study, NO metabolites, nitrite and nitrate, were quantified in RBCs and whole blood with time of storage. Whole blood (WB), leukoreduced (LR), and non-leukoreduced (NLR) components were obtained from healthy volunteer donors and stored in polyvinyl chloride bags for 42 days. Nitrite and nitrate were measured using reductive gas-phase chemiluminescence.

Results: Nitrite concentrations initially decreased rapidly from about 150nmol/L, but stabilized at about 44nmol/L in room air for up to 42 days. Nitrate concentrations remained stable during storage at about 35µmol/L. Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air. Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.

Conclusion: As erythrocytes may contribute to the control of blood flow and oxygen delivery through reduction of nitrite to NO under hypoxic conditions, the present findings provide insight into possible effects of blood transfusion. These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

No MeSH data available.


Related in: MedlinePlus

Effect of storage on nitrate concentration in blood stored for 42 days in room air (4A) or an argon chamber (4B); number of donors, n=3 (A), n=3 (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3814924&req=5

f4: Effect of storage on nitrate concentration in blood stored for 42 days in room air (4A) or an argon chamber (4B); number of donors, n=3 (A), n=3 (B).

Mentions: In contrast to the results with nitrite ions, nitrate levels in WB, in NLR and LR RBCs, and in supernatant samples remained steady for the duration of blood storage (Figure 4). Whole blood nitrate levels were slightly higher than either the non-leukoreduced or the leukoreduced RBC components stored either in air or in the argon chamber. In room air, WB nitrate concentration was about 47 ± 2µM, while NLR and LR nitrate concentrations were about 34 ± 2µM. For blood stored in the argon chamber, WB nitrate levels were lower than respective room air samples, with chamber nitrate remaining at about 37 ± 3µM. LR and NLR RBC nitrate levels in the chamber were unchanged when compared to LR and NLR RBCs stored in air, remaining at about 35 ± 2µM. Supernatant nitrate levels (measured after centrifugation of packed red cells or whole blood) followed the same trend as the blood samples, remaining steady for the duration of storage and exhibiting similar nitrate concentrations when stored in an argon chamber (data not shown).


Effect of storage levels of nitric oxide derivatives in blood components.

Qazi MA, Rizzatti F, Piknova B, Sibmooh N, Stroncek DF, Schechter AN - F1000Res (2012)

Effect of storage on nitrate concentration in blood stored for 42 days in room air (4A) or an argon chamber (4B); number of donors, n=3 (A), n=3 (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814924&req=5

f4: Effect of storage on nitrate concentration in blood stored for 42 days in room air (4A) or an argon chamber (4B); number of donors, n=3 (A), n=3 (B).
Mentions: In contrast to the results with nitrite ions, nitrate levels in WB, in NLR and LR RBCs, and in supernatant samples remained steady for the duration of blood storage (Figure 4). Whole blood nitrate levels were slightly higher than either the non-leukoreduced or the leukoreduced RBC components stored either in air or in the argon chamber. In room air, WB nitrate concentration was about 47 ± 2µM, while NLR and LR nitrate concentrations were about 34 ± 2µM. For blood stored in the argon chamber, WB nitrate levels were lower than respective room air samples, with chamber nitrate remaining at about 37 ± 3µM. LR and NLR RBC nitrate levels in the chamber were unchanged when compared to LR and NLR RBCs stored in air, remaining at about 35 ± 2µM. Supernatant nitrate levels (measured after centrifugation of packed red cells or whole blood) followed the same trend as the blood samples, remaining steady for the duration of storage and exhibiting similar nitrate concentrations when stored in an argon chamber (data not shown).

Bottom Line: Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air.Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

View Article: PubMed Central - PubMed

Affiliation: Molecular Medicine Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

ABSTRACT

Background: Potential deleterious effects of red blood cell (RBC) transfusions, especially from blood kept at length, have been ascribed to biochemical changes during storage, including those of nitric oxide (NO) metabolism. Study methods and design: In this study, NO metabolites, nitrite and nitrate, were quantified in RBCs and whole blood with time of storage. Whole blood (WB), leukoreduced (LR), and non-leukoreduced (NLR) components were obtained from healthy volunteer donors and stored in polyvinyl chloride bags for 42 days. Nitrite and nitrate were measured using reductive gas-phase chemiluminescence.

Results: Nitrite concentrations initially decreased rapidly from about 150nmol/L, but stabilized at about 44nmol/L in room air for up to 42 days. Nitrate concentrations remained stable during storage at about 35µmol/L. Cells from bags maintained in an argon chamber showed decreased nitrite levels compared to those maintained in room air. Inhibition of enzymes implicated in the NO cycle did not alter nitrite levels.

Conclusion: As erythrocytes may contribute to the control of blood flow and oxygen delivery through reduction of nitrite to NO under hypoxic conditions, the present findings provide insight into possible effects of blood transfusion. These measurements may explain some adverse effects of RBC transfusion and suggest ways of optimizing the preservation of stored blood.

No MeSH data available.


Related in: MedlinePlus