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Multispectral Photoacoustic Imaging of Prostate Cancer: Preliminary Ex-vivo Results.

Dogra VS, Chinni BK, Valluru KS, Joseph JV, Ghazi A, Yao JL, Evans K, Messing EM, Rao NA - J Clin Imaging Sci (2013)

Bottom Line: A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent.Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively.Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Urology, and BME, University of Rochester, Rochester, NY 14642, USA.

ABSTRACT

Objective: The objective of this study is to validate if ex-vivo multispectral photoacoustic (PA) imaging can differentiate between malignant prostate tissue, benign prostatic hyperplasia (BPH), and normal human prostate tissue.

Materials and methods: Institutional Review Board's approval was obtained for this study. A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent. Multispectral PA imaging was performed on surgically excised prostate tissue and chromophore images that represent optical absorption of deoxyhemoglobin (dHb), oxyhemoglobin (HbO2), lipid, and water were reconstructed. After the imaging procedure is completed, malignant prostate, BPH and normal prostate regions were marked by the genitourinary pathologist on histopathology slides and digital images of marked histopathology slides were obtained. The histopathology images were co-registered with chromophore images. Region of interest (ROI) corresponding to malignant prostate, BPH and normal prostate were defined on the chromophore images. Pixel values within each ROI were then averaged to determine mean intensities of dHb, HbO2, lipid, and water.

Results: Our preliminary results show that there is statistically significant difference in mean intensity of dHb (P < 0.0001) and lipid (P = 0.0251) between malignant prostate and normal prostate tissue. There was difference in mean intensity of dHb (P < 0.0001) between malignant prostate and BPH. Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively.

Conclusion: Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.

No MeSH data available.


Related in: MedlinePlus

Differences between C-scan and B-scan image formation. B-scan image depicts sagittal or transverse planes in the body where as a C-scan image depicts information from coronal plane in the body. Reproduced with permission from “Basics and Clinical Applications of Photoacoustic Imaging,” Ultrasound Clinics, Vol. 4, Issue 3: 403-429, July 2009.
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Figure 5: Differences between C-scan and B-scan image formation. B-scan image depicts sagittal or transverse planes in the body where as a C-scan image depicts information from coronal plane in the body. Reproduced with permission from “Basics and Clinical Applications of Photoacoustic Imaging,” Ultrasound Clinics, Vol. 4, Issue 3: 403-429, July 2009.

Mentions: C-scan is an image produced by spatially sampling the ultrasound signal amplitude at a fixed time while the sensor is laterally scanned over a tissue surface.[14] The C-scan image is vividly different from a conventional B-scan image generated by an ultrasound imaging system in that C-scan image depicts information in the coronal plane, whereas a B-scan image conveys information from sagittal or transverse plane in the body as shown in Figure 5.


Multispectral Photoacoustic Imaging of Prostate Cancer: Preliminary Ex-vivo Results.

Dogra VS, Chinni BK, Valluru KS, Joseph JV, Ghazi A, Yao JL, Evans K, Messing EM, Rao NA - J Clin Imaging Sci (2013)

Differences between C-scan and B-scan image formation. B-scan image depicts sagittal or transverse planes in the body where as a C-scan image depicts information from coronal plane in the body. Reproduced with permission from “Basics and Clinical Applications of Photoacoustic Imaging,” Ultrasound Clinics, Vol. 4, Issue 3: 403-429, July 2009.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814905&req=5

Figure 5: Differences between C-scan and B-scan image formation. B-scan image depicts sagittal or transverse planes in the body where as a C-scan image depicts information from coronal plane in the body. Reproduced with permission from “Basics and Clinical Applications of Photoacoustic Imaging,” Ultrasound Clinics, Vol. 4, Issue 3: 403-429, July 2009.
Mentions: C-scan is an image produced by spatially sampling the ultrasound signal amplitude at a fixed time while the sensor is laterally scanned over a tissue surface.[14] The C-scan image is vividly different from a conventional B-scan image generated by an ultrasound imaging system in that C-scan image depicts information in the coronal plane, whereas a B-scan image conveys information from sagittal or transverse plane in the body as shown in Figure 5.

Bottom Line: A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent.Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively.Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Urology, and BME, University of Rochester, Rochester, NY 14642, USA.

ABSTRACT

Objective: The objective of this study is to validate if ex-vivo multispectral photoacoustic (PA) imaging can differentiate between malignant prostate tissue, benign prostatic hyperplasia (BPH), and normal human prostate tissue.

Materials and methods: Institutional Review Board's approval was obtained for this study. A total of 30 patients undergoing prostatectomy for biopsy-confirmed prostate cancer were included in this study with informed consent. Multispectral PA imaging was performed on surgically excised prostate tissue and chromophore images that represent optical absorption of deoxyhemoglobin (dHb), oxyhemoglobin (HbO2), lipid, and water were reconstructed. After the imaging procedure is completed, malignant prostate, BPH and normal prostate regions were marked by the genitourinary pathologist on histopathology slides and digital images of marked histopathology slides were obtained. The histopathology images were co-registered with chromophore images. Region of interest (ROI) corresponding to malignant prostate, BPH and normal prostate were defined on the chromophore images. Pixel values within each ROI were then averaged to determine mean intensities of dHb, HbO2, lipid, and water.

Results: Our preliminary results show that there is statistically significant difference in mean intensity of dHb (P < 0.0001) and lipid (P = 0.0251) between malignant prostate and normal prostate tissue. There was difference in mean intensity of dHb (P < 0.0001) between malignant prostate and BPH. Sensitivity, specificity, positive predictive value, and negative predictive value of our imaging system were found to be 81.3%, 96.2%, 92.9% and 89.3% respectively.

Conclusion: Our preliminary results of ex-vivo human prostate study suggest that multispectral PA imaging can differentiate between malignant prostate, BPH and normal prostate tissue.

No MeSH data available.


Related in: MedlinePlus