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Lithium carbonate inducing disorders in three parameters of rat sperm.

Toghyani S, Dashti GR, Roudbari NH, Rouzbehani S, Monajemi R - Adv Biomed Res (2013)

Bottom Line: Lithium has a significant impact in reducing the symptoms of bipolar mania but in long periods of use with therapeutic doses can cause several disorders in various organs including the reproductive system.At the end of this period, sperm cells isolated from the cauda epididymis were counted, motility was estimated, and stained with smear papanicolaou stain.Lithium alters intracellular signaling pathways such as inositol phosphate metabolic cycle and cyclic adenosine mono phosphate (cAMP) system and adenosine triphosphate (ATP) synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Islamic Azad University, Tehran Science and Research University, Isfahan, Iran.

ABSTRACT

Background: Lithium has a significant impact in reducing the symptoms of bipolar mania but in long periods of use with therapeutic doses can cause several disorders in various organs including the reproductive system. In this study, the effect of lithium on the sperm concentration and motility and forms of abnormal cells has been examined.

Materials and methods: Male Wistar rats under the 48-day treatment with lithium carbonate at doses of 10, 20, and 30 mg/kg bw/day were kept in standard conditions. At the end of this period, sperm cells isolated from the cauda epididymis were counted, motility was estimated, and stained with smear papanicolaou stain.

Results: In lithium-treated groups, the rate of spermatogenesis and sperm quality were reduced and was seen in a dose-dependent manner.

Discussion: Lithium alters intracellular signaling pathways such as inositol phosphate metabolic cycle and cyclic adenosine mono phosphate (cAMP) system and adenosine triphosphate (ATP) synthesis. It also interferes in the division of sex cells to produce mature sperm and showed changes in the sperm cell membrane, function, and structure.

No MeSH data available.


Related in: MedlinePlus

Papanicolaou staining of sperm cells of the group treated with 30 mg lithium carbonate under a light microscope with magnification of ×40 showed abnormal curling tails with abnormal sperms, which was more than that of 10 mg and 20 mg doses, and the least sperm density was observed using 30 mg dose
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Figure 7: Papanicolaou staining of sperm cells of the group treated with 30 mg lithium carbonate under a light microscope with magnification of ×40 showed abnormal curling tails with abnormal sperms, which was more than that of 10 mg and 20 mg doses, and the least sperm density was observed using 30 mg dose

Mentions: Studies have shown that men using lithium for a long time suffer from complications such as reduced steroidogenic activity and reduced efficiency of spermatogenesis process.[13] In a study on a species of Viscacha (Lagostomus maximus maximus), a 35-day injection of therapeutic doses of lithium showed destruction of Leydig cells followed by reduction of plasma testosterone level and remarkable reduction of sperm motility and viability in sperm solution.[14] Lithium reduces the activity of hypothalamus-pituitary-gonad axis, and spermatogenesis-stimulating hormones. It also affects testicles directly that may result in profound complications. Since the lithium ion can pass through the blood-testis barrier, it affects developing sexual cells and disrupts maturation and release of spermatozoa out of seminiferous epithelium by stopping cell differentiation and growth cycle and consequently reduces the number of the total sperm count.[1113] The results of this study also confirm the role of lithium in reduction of the produced sperm, number of motile sperms, and motility of sperm's tail and production of abnormal sperms. Papanicolaou staining showed sperms with abnormal tail. These results agree with the results of the previous studies on the effect of lithium on the function of male reproductive system. Some probable reasons for verifying results of this study will be mentioned in the following parts [Figures 4–7].


Lithium carbonate inducing disorders in three parameters of rat sperm.

Toghyani S, Dashti GR, Roudbari NH, Rouzbehani S, Monajemi R - Adv Biomed Res (2013)

Papanicolaou staining of sperm cells of the group treated with 30 mg lithium carbonate under a light microscope with magnification of ×40 showed abnormal curling tails with abnormal sperms, which was more than that of 10 mg and 20 mg doses, and the least sperm density was observed using 30 mg dose
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814897&req=5

Figure 7: Papanicolaou staining of sperm cells of the group treated with 30 mg lithium carbonate under a light microscope with magnification of ×40 showed abnormal curling tails with abnormal sperms, which was more than that of 10 mg and 20 mg doses, and the least sperm density was observed using 30 mg dose
Mentions: Studies have shown that men using lithium for a long time suffer from complications such as reduced steroidogenic activity and reduced efficiency of spermatogenesis process.[13] In a study on a species of Viscacha (Lagostomus maximus maximus), a 35-day injection of therapeutic doses of lithium showed destruction of Leydig cells followed by reduction of plasma testosterone level and remarkable reduction of sperm motility and viability in sperm solution.[14] Lithium reduces the activity of hypothalamus-pituitary-gonad axis, and spermatogenesis-stimulating hormones. It also affects testicles directly that may result in profound complications. Since the lithium ion can pass through the blood-testis barrier, it affects developing sexual cells and disrupts maturation and release of spermatozoa out of seminiferous epithelium by stopping cell differentiation and growth cycle and consequently reduces the number of the total sperm count.[1113] The results of this study also confirm the role of lithium in reduction of the produced sperm, number of motile sperms, and motility of sperm's tail and production of abnormal sperms. Papanicolaou staining showed sperms with abnormal tail. These results agree with the results of the previous studies on the effect of lithium on the function of male reproductive system. Some probable reasons for verifying results of this study will be mentioned in the following parts [Figures 4–7].

Bottom Line: Lithium has a significant impact in reducing the symptoms of bipolar mania but in long periods of use with therapeutic doses can cause several disorders in various organs including the reproductive system.At the end of this period, sperm cells isolated from the cauda epididymis were counted, motility was estimated, and stained with smear papanicolaou stain.Lithium alters intracellular signaling pathways such as inositol phosphate metabolic cycle and cyclic adenosine mono phosphate (cAMP) system and adenosine triphosphate (ATP) synthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Islamic Azad University, Tehran Science and Research University, Isfahan, Iran.

ABSTRACT

Background: Lithium has a significant impact in reducing the symptoms of bipolar mania but in long periods of use with therapeutic doses can cause several disorders in various organs including the reproductive system. In this study, the effect of lithium on the sperm concentration and motility and forms of abnormal cells has been examined.

Materials and methods: Male Wistar rats under the 48-day treatment with lithium carbonate at doses of 10, 20, and 30 mg/kg bw/day were kept in standard conditions. At the end of this period, sperm cells isolated from the cauda epididymis were counted, motility was estimated, and stained with smear papanicolaou stain.

Results: In lithium-treated groups, the rate of spermatogenesis and sperm quality were reduced and was seen in a dose-dependent manner.

Discussion: Lithium alters intracellular signaling pathways such as inositol phosphate metabolic cycle and cyclic adenosine mono phosphate (cAMP) system and adenosine triphosphate (ATP) synthesis. It also interferes in the division of sex cells to produce mature sperm and showed changes in the sperm cell membrane, function, and structure.

No MeSH data available.


Related in: MedlinePlus