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Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry.

Cui L, Meng Y, Xu D, Feng Y, Chen G, Hu B, Feng G, Yin L - Ther Clin Risk Manag (2013)

Bottom Line: Orthogonal signal correction-partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (-) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (-) group.However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (-) group.There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide.

View Article: PubMed Central - PubMed

Affiliation: Xi'xiang People's Hospital Affiliated to Guangdong Medical College, Shenzhen, People's Republic of China.

ABSTRACT
The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (-) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction-partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (-) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (-) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (-) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress.

No MeSH data available.


(A) Orthogonal signal correction–partial least squares discriminate analysis score plots of the three groups after hemodialysis. The ellipse shows the 95% confidence region for Hotelling’s T2 statistic for this model. Cumulative fitness (values) and prediction power (value) of this three-component model were 0.915 and 0.952, respectively. 1, G (−) group; 2, G (+) group; 3, control group. (B) Orthogonal partial least squares S-plot based on detected plasma metabolites from the G (−) and G (+) groups.Notes: The G (−) group was treated with glucose-free dialysate, and the G (+) group was treated with glucose-added dialysate.
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f3-tcrm-9-417: (A) Orthogonal signal correction–partial least squares discriminate analysis score plots of the three groups after hemodialysis. The ellipse shows the 95% confidence region for Hotelling’s T2 statistic for this model. Cumulative fitness (values) and prediction power (value) of this three-component model were 0.915 and 0.952, respectively. 1, G (−) group; 2, G (+) group; 3, control group. (B) Orthogonal partial least squares S-plot based on detected plasma metabolites from the G (−) and G (+) groups.Notes: The G (−) group was treated with glucose-free dialysate, and the G (+) group was treated with glucose-added dialysate.

Mentions: Principal component analysis (Figure 2) revealed distinctive boundaries in the pre-dialysis specimens between the healthy control group and the MHD groups. The specimens from the healthy control group were better concentrated, with good metabolite consistency, and the specimens of the MHD groups were mostly dispersed with large differences. The pre-dialysis specimens of the G (−) and G (+) groups could not be separated by PCA analysis. The same situations occurred in the PLS-DA analysis (Figure 3), such that all pre-dialysis metabolic states of all MHD subjects could not be consistently separated.


Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry.

Cui L, Meng Y, Xu D, Feng Y, Chen G, Hu B, Feng G, Yin L - Ther Clin Risk Manag (2013)

(A) Orthogonal signal correction–partial least squares discriminate analysis score plots of the three groups after hemodialysis. The ellipse shows the 95% confidence region for Hotelling’s T2 statistic for this model. Cumulative fitness (values) and prediction power (value) of this three-component model were 0.915 and 0.952, respectively. 1, G (−) group; 2, G (+) group; 3, control group. (B) Orthogonal partial least squares S-plot based on detected plasma metabolites from the G (−) and G (+) groups.Notes: The G (−) group was treated with glucose-free dialysate, and the G (+) group was treated with glucose-added dialysate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814896&req=5

f3-tcrm-9-417: (A) Orthogonal signal correction–partial least squares discriminate analysis score plots of the three groups after hemodialysis. The ellipse shows the 95% confidence region for Hotelling’s T2 statistic for this model. Cumulative fitness (values) and prediction power (value) of this three-component model were 0.915 and 0.952, respectively. 1, G (−) group; 2, G (+) group; 3, control group. (B) Orthogonal partial least squares S-plot based on detected plasma metabolites from the G (−) and G (+) groups.Notes: The G (−) group was treated with glucose-free dialysate, and the G (+) group was treated with glucose-added dialysate.
Mentions: Principal component analysis (Figure 2) revealed distinctive boundaries in the pre-dialysis specimens between the healthy control group and the MHD groups. The specimens from the healthy control group were better concentrated, with good metabolite consistency, and the specimens of the MHD groups were mostly dispersed with large differences. The pre-dialysis specimens of the G (−) and G (+) groups could not be separated by PCA analysis. The same situations occurred in the PLS-DA analysis (Figure 3), such that all pre-dialysis metabolic states of all MHD subjects could not be consistently separated.

Bottom Line: Orthogonal signal correction-partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (-) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (-) group.However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (-) group.There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide.

View Article: PubMed Central - PubMed

Affiliation: Xi'xiang People's Hospital Affiliated to Guangdong Medical College, Shenzhen, People's Republic of China.

ABSTRACT
The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (-) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction-partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (-) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (-) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (-) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress.

No MeSH data available.