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Detection of Salmonella by Surface Plasmon Resonance

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.


Sensograms showing: (a) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:4 detection antibody, which is specific for Salmonella Typhimurium. (b) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:9 detection antibody, which is specific for Salmonella Enteritidis. Both antibodies show no cross-reactivity to E. coli K12 (0 AU).
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f5-sensors-07-01427: Sensograms showing: (a) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:4 detection antibody, which is specific for Salmonella Typhimurium. (b) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:9 detection antibody, which is specific for Salmonella Enteritidis. Both antibodies show no cross-reactivity to E. coli K12 (0 AU).

Mentions: Cross-reactivity of the O:4 detection antibody to Salmonella Enteritidis (Fig. 4b) and O:9 detection antibody to Salmonella Typhimurium was also evaluated (Fig. 3b). As expected, the antibodies were found to be specific for the respective serovars. Furthermore, no cross-reactivity was observed when milk spiked with E. coli K12 (1.0×109 cells mL−1) was probed using the O-specific antibodies (Fig. 5).


Detection of Salmonella by Surface Plasmon Resonance
Sensograms showing: (a) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:4 detection antibody, which is specific for Salmonella Typhimurium. (b) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:9 detection antibody, which is specific for Salmonella Enteritidis. Both antibodies show no cross-reactivity to E. coli K12 (0 AU).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814861&req=5

f5-sensors-07-01427: Sensograms showing: (a) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:4 detection antibody, which is specific for Salmonella Typhimurium. (b) Cross-reactivity check using spiked milk containing E. coli K12 (1.0×109 cells mL−1) against O:9 detection antibody, which is specific for Salmonella Enteritidis. Both antibodies show no cross-reactivity to E. coli K12 (0 AU).
Mentions: Cross-reactivity of the O:4 detection antibody to Salmonella Enteritidis (Fig. 4b) and O:9 detection antibody to Salmonella Typhimurium was also evaluated (Fig. 3b). As expected, the antibodies were found to be specific for the respective serovars. Furthermore, no cross-reactivity was observed when milk spiked with E. coli K12 (1.0×109 cells mL−1) was probed using the O-specific antibodies (Fig. 5).

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.