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Detection of Salmonella by Surface Plasmon Resonance

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.


Detection range of SPR-based assay for specific detection of Salmonella Enteritidis in PBS buffer system using O-specific O:9 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:9 detection antibody.
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f2-sensors-07-01427: Detection range of SPR-based assay for specific detection of Salmonella Enteritidis in PBS buffer system using O-specific O:9 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:9 detection antibody.

Mentions: In case of Salmonella Enteritidis the LLD of the assay using the O:9 detection antibody was much higher compared to that of the Salmonella Typhimurium. In this case the LLD was 2.5×108 cells mL-1 corresponding to a detection signal of 29 ± 4.3 AU. The signal obtained from the highest concentration (2.5×109 cells mL−1) of Salmonella Enteritidis was 68 ± 5.4 AU (Fig. 2).


Detection of Salmonella by Surface Plasmon Resonance
Detection range of SPR-based assay for specific detection of Salmonella Enteritidis in PBS buffer system using O-specific O:9 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:9 detection antibody.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814861&req=5

f2-sensors-07-01427: Detection range of SPR-based assay for specific detection of Salmonella Enteritidis in PBS buffer system using O-specific O:9 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:9 detection antibody.
Mentions: In case of Salmonella Enteritidis the LLD of the assay using the O:9 detection antibody was much higher compared to that of the Salmonella Typhimurium. In this case the LLD was 2.5×108 cells mL-1 corresponding to a detection signal of 29 ± 4.3 AU. The signal obtained from the highest concentration (2.5×109 cells mL−1) of Salmonella Enteritidis was 68 ± 5.4 AU (Fig. 2).

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.