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Detection of Salmonella by Surface Plasmon Resonance

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.


Detection range of SPR-based assay for specific detection of Salmonella Typhimurium in PBS buffer system using O-specific O:4 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:4 detection antibody.
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f1-sensors-07-01427: Detection range of SPR-based assay for specific detection of Salmonella Typhimurium in PBS buffer system using O-specific O:4 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:4 detection antibody.

Mentions: Different concentrations of both the Salmonella serovars were evaluated separately using the assay in PBS. Salmonella Typhimurium was found to have a lower limit of detection (LLD, defined as the concentration of cells resulting in a detection signal, which is the average value of the detection signal obtained due to control plus 3 times the standard deviation) of 1.25×105 cells mL−1 (47 ± 3.9 AU) when probed with the O:4 detection antibody. The highest concentration of Salmonella Typhimurium in PBS evaluated was 2.5×106 cells mL−1 resulting in a detection signal of 101 ± 8.3 AU (Fig. 1).


Detection of Salmonella by Surface Plasmon Resonance
Detection range of SPR-based assay for specific detection of Salmonella Typhimurium in PBS buffer system using O-specific O:4 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:4 detection antibody.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814861&req=5

f1-sensors-07-01427: Detection range of SPR-based assay for specific detection of Salmonella Typhimurium in PBS buffer system using O-specific O:4 detection antibody. Note that the plot is semi-logarithmic with the cell concentrations increasing exponentially on the X-axis. The signals represented here are those obtained after addition of the O:4 detection antibody.
Mentions: Different concentrations of both the Salmonella serovars were evaluated separately using the assay in PBS. Salmonella Typhimurium was found to have a lower limit of detection (LLD, defined as the concentration of cells resulting in a detection signal, which is the average value of the detection signal obtained due to control plus 3 times the standard deviation) of 1.25×105 cells mL−1 (47 ± 3.9 AU) when probed with the O:4 detection antibody. The highest concentration of Salmonella Typhimurium in PBS evaluated was 2.5×106 cells mL−1 resulting in a detection signal of 101 ± 8.3 AU (Fig. 1).

View Article: PubMed Central

ABSTRACT

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL−1 and 2.50×108 cells mL−1, respectively.

No MeSH data available.