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Raman Spectroscopy Cell-based Biosensors

View Article: PubMed Central

ABSTRACT

One of the main challenges faced by biodetection systems is the ability to detect and identify a large range of toxins at low concentrations and in short times. Cell-based biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic agents. Raman spectroscopy is a powerful technique for studying cellular biochemistry. Different toxic chemicals have different effects on living cells and induce different time-dependent biochemical changes related to cell death mechanisms. Cellular changes start with membrane receptor signalling leading to cytoplasmic shrinkage and nuclear fragmentation. The potential advantage of Raman spectroscopy cell-based systems is that they are not engineered to respond specifically to a single toxic agent but are free to react to many biologically active compounds. Raman spectroscopy biosensors can also provide additional information from the time-dependent changes of cellular biochemistry. Since no cell labelling or staining is required, the specific time dependent biochemical changes in the living cells can be used for the identification and quantification of the toxic agents. Thus, detection of biochemical changes of cells by Raman spectroscopy could overcome the limitations of other biosensor techniques, with respect to detection and discrimination of a large range of toxic agents. Further developments of this technique may also include integration of cellular microarrays for high throughput in vitro toxicological testing of pharmaceuticals and in situ monitoring of the growth of engineered tissues.

No MeSH data available.


Difference between the Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (Ricin 10 nM) and sulphur mustard (S.M. at 200 μM, 500 μM and 1000 μM).
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f5-sensors-07-01343: Difference between the Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (Ricin 10 nM) and sulphur mustard (S.M. at 200 μM, 500 μM and 1000 μM).

Mentions: Differences between Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (10 nM) and SM (200-1000 μM) are shown in Figure 5 [37].


Raman Spectroscopy Cell-based Biosensors
Difference between the Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (Ricin 10 nM) and sulphur mustard (S.M. at 200 μM, 500 μM and 1000 μM).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814856&req=5

f5-sensors-07-01343: Difference between the Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (Ricin 10 nM) and sulphur mustard (S.M. at 200 μM, 500 μM and 1000 μM).
Mentions: Differences between Raman spectra of healthy A549 cells and A549 cells exposed for 24 hours to ricin (10 nM) and SM (200-1000 μM) are shown in Figure 5 [37].

View Article: PubMed Central

ABSTRACT

One of the main challenges faced by biodetection systems is the ability to detect and identify a large range of toxins at low concentrations and in short times. Cell-based biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic agents. Raman spectroscopy is a powerful technique for studying cellular biochemistry. Different toxic chemicals have different effects on living cells and induce different time-dependent biochemical changes related to cell death mechanisms. Cellular changes start with membrane receptor signalling leading to cytoplasmic shrinkage and nuclear fragmentation. The potential advantage of Raman spectroscopy cell-based systems is that they are not engineered to respond specifically to a single toxic agent but are free to react to many biologically active compounds. Raman spectroscopy biosensors can also provide additional information from the time-dependent changes of cellular biochemistry. Since no cell labelling or staining is required, the specific time dependent biochemical changes in the living cells can be used for the identification and quantification of the toxic agents. Thus, detection of biochemical changes of cells by Raman spectroscopy could overcome the limitations of other biosensor techniques, with respect to detection and discrimination of a large range of toxic agents. Further developments of this technique may also include integration of cellular microarrays for high throughput in vitro toxicological testing of pharmaceuticals and in situ monitoring of the growth of engineered tissues.

No MeSH data available.