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Cytogenetic effects of 1,1-dichloroethane in mice bone marrow cells.

Patlolla BP, Patlolla AK, Tchounwou PB - Int J Environ Res Public Health (2005)

Bottom Line: Several chlorinated alkanes and alkenes were found to induce toxic effects.All animals were sacrificed 24 hours after the treatment.Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Alcorn State University, Lorman, MS, USA. babu@lorman.alcorn.edu

ABSTRACT
The major concern for the halogenated compounds is their widespread distribution, in addition to occupational exposures. Several chlorinated alkanes and alkenes were found to induce toxic effects. In this study, we investigated the genotoxic potential of 1,1-dichloroethane in the bone marrow cells obtained from Swiss-Webster mice, using chromosomal aberrations (CA), mitotic index (MI), and micronuclei (MN) formation as toxicological endpoints. Five groups of three male mice each, weighing an average of 24 +/- 2 g, were injected intraperitoneally, once with doses of 100, 200, 300, 400, 500 mg/kg body weight (BW) of 1,1-dichloroethane dissolved in ethanol. A control group was also made of three animals injected with ethanol (1%) without the chemical. All animals were sacrificed 24 hours after the treatment. Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols. Chromatid and chromosome aberrations were investigated in 100 metaphase cells per animal and percent micronuclei frequencies were investigated in 1,000 metaphase cells per animal. 1,1-dichloroethane exposures significantly increased the number of chromosomal aberrations and the frequency of micronucleated cells in the bone marrow cells of Swiss-Webster mice. Percent chromosomal aberrations of 2.67 +/- 0.577, 7.66 +/- 2.89, 8.33 +/- 2.08, 14.67 +/- 2.51, 20.3 +/- 3.21, 28 +/- 3.61; mitotic index of 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% and micronuclei frequencies of 3.33 +/- 0.7, 7.33 +/- 0.9, 8.00 +/- 1.0, 11.67 +/- 1.2, 15.33 +/- 0.7, 18.00 +/- 1.7 were recorded for the control, 100, 200, 300, 400, and 500 mg/kg BW respectively; indicating a gradual increase in number of chromosomal aberrations and micronuclei formation, with increasing dose of 1,1,-dichloroethane. Our results indicate that 1,1-dichloroethane has a genotoxic potential as measured by the bone marrow CA and MN tests in Swiss-Webster mice.

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Effect of 1,1-Dichloroethane on the percent Mitotic Index.
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f2-ijerph-02-00101: Effect of 1,1-Dichloroethane on the percent Mitotic Index.

Mentions: The mitotic index was used to determine the rate of cell division. The slides prepared for the assessment of chromosomal aberrations were used for calculating the mitotic index. Mitotic Index depression was observed in the bone marrow cells of mice as compared with the control, it was found to be dose-dependent (Figure 2). Mean percentages of mitotic index were 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% for the doses 0, 100, 200, 300, 400 and 500 mg/Kg BW of 1,1-Dichloroethane respectively.


Cytogenetic effects of 1,1-dichloroethane in mice bone marrow cells.

Patlolla BP, Patlolla AK, Tchounwou PB - Int J Environ Res Public Health (2005)

Effect of 1,1-Dichloroethane on the percent Mitotic Index.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814703&req=5

f2-ijerph-02-00101: Effect of 1,1-Dichloroethane on the percent Mitotic Index.
Mentions: The mitotic index was used to determine the rate of cell division. The slides prepared for the assessment of chromosomal aberrations were used for calculating the mitotic index. Mitotic Index depression was observed in the bone marrow cells of mice as compared with the control, it was found to be dose-dependent (Figure 2). Mean percentages of mitotic index were 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% for the doses 0, 100, 200, 300, 400 and 500 mg/Kg BW of 1,1-Dichloroethane respectively.

Bottom Line: Several chlorinated alkanes and alkenes were found to induce toxic effects.All animals were sacrificed 24 hours after the treatment.Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Alcorn State University, Lorman, MS, USA. babu@lorman.alcorn.edu

ABSTRACT
The major concern for the halogenated compounds is their widespread distribution, in addition to occupational exposures. Several chlorinated alkanes and alkenes were found to induce toxic effects. In this study, we investigated the genotoxic potential of 1,1-dichloroethane in the bone marrow cells obtained from Swiss-Webster mice, using chromosomal aberrations (CA), mitotic index (MI), and micronuclei (MN) formation as toxicological endpoints. Five groups of three male mice each, weighing an average of 24 +/- 2 g, were injected intraperitoneally, once with doses of 100, 200, 300, 400, 500 mg/kg body weight (BW) of 1,1-dichloroethane dissolved in ethanol. A control group was also made of three animals injected with ethanol (1%) without the chemical. All animals were sacrificed 24 hours after the treatment. Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols. Chromatid and chromosome aberrations were investigated in 100 metaphase cells per animal and percent micronuclei frequencies were investigated in 1,000 metaphase cells per animal. 1,1-dichloroethane exposures significantly increased the number of chromosomal aberrations and the frequency of micronucleated cells in the bone marrow cells of Swiss-Webster mice. Percent chromosomal aberrations of 2.67 +/- 0.577, 7.66 +/- 2.89, 8.33 +/- 2.08, 14.67 +/- 2.51, 20.3 +/- 3.21, 28 +/- 3.61; mitotic index of 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% and micronuclei frequencies of 3.33 +/- 0.7, 7.33 +/- 0.9, 8.00 +/- 1.0, 11.67 +/- 1.2, 15.33 +/- 0.7, 18.00 +/- 1.7 were recorded for the control, 100, 200, 300, 400, and 500 mg/kg BW respectively; indicating a gradual increase in number of chromosomal aberrations and micronuclei formation, with increasing dose of 1,1,-dichloroethane. Our results indicate that 1,1-dichloroethane has a genotoxic potential as measured by the bone marrow CA and MN tests in Swiss-Webster mice.

Show MeSH
Related in: MedlinePlus