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Cytogenetic effects of 1,1-dichloroethane in mice bone marrow cells.

Patlolla BP, Patlolla AK, Tchounwou PB - Int J Environ Res Public Health (2005)

Bottom Line: Several chlorinated alkanes and alkenes were found to induce toxic effects.All animals were sacrificed 24 hours after the treatment.Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Alcorn State University, Lorman, MS, USA. babu@lorman.alcorn.edu

ABSTRACT
The major concern for the halogenated compounds is their widespread distribution, in addition to occupational exposures. Several chlorinated alkanes and alkenes were found to induce toxic effects. In this study, we investigated the genotoxic potential of 1,1-dichloroethane in the bone marrow cells obtained from Swiss-Webster mice, using chromosomal aberrations (CA), mitotic index (MI), and micronuclei (MN) formation as toxicological endpoints. Five groups of three male mice each, weighing an average of 24 +/- 2 g, were injected intraperitoneally, once with doses of 100, 200, 300, 400, 500 mg/kg body weight (BW) of 1,1-dichloroethane dissolved in ethanol. A control group was also made of three animals injected with ethanol (1%) without the chemical. All animals were sacrificed 24 hours after the treatment. Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols. Chromatid and chromosome aberrations were investigated in 100 metaphase cells per animal and percent micronuclei frequencies were investigated in 1,000 metaphase cells per animal. 1,1-dichloroethane exposures significantly increased the number of chromosomal aberrations and the frequency of micronucleated cells in the bone marrow cells of Swiss-Webster mice. Percent chromosomal aberrations of 2.67 +/- 0.577, 7.66 +/- 2.89, 8.33 +/- 2.08, 14.67 +/- 2.51, 20.3 +/- 3.21, 28 +/- 3.61; mitotic index of 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% and micronuclei frequencies of 3.33 +/- 0.7, 7.33 +/- 0.9, 8.00 +/- 1.0, 11.67 +/- 1.2, 15.33 +/- 0.7, 18.00 +/- 1.7 were recorded for the control, 100, 200, 300, 400, and 500 mg/kg BW respectively; indicating a gradual increase in number of chromosomal aberrations and micronuclei formation, with increasing dose of 1,1,-dichloroethane. Our results indicate that 1,1-dichloroethane has a genotoxic potential as measured by the bone marrow CA and MN tests in Swiss-Webster mice.

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Effect of 1,1-Dichloroethane on the frequency of chromosomal aberrations.
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f1-ijerph-02-00101: Effect of 1,1-Dichloroethane on the frequency of chromosomal aberrations.

Mentions: 1,1-Dichloroethane (DCE) induced a statistically significant increase in chromosome aberrations in metaphase bone marrow cells. Table. 1 shows the mean frequency of cells with aberrations (as percentages) calculated for each dose with 3 animals/dose. The most frequent types of aberrations were gaps, breaks and fragments. Chromatid-type aberrations were detected at high frequencies. Relatively higher frequencies of gaps were observed for all the doses tested. A quantitative assessment of the distribution of breaks and gaps revealed that the distal regions of the chromosomes were more vulnerable to the effects of 1,1-Dichloroethane. The frequency of chromosomal aberrations also increased with increasing doses of 1,1-dichloroethane (Figure 1). The mean percentages of the induced chromosomal aberrations were 2.67 ± 0.577 %, 7.66 ± 2.89 %, 8.33 ± 2.08 %, 14.67 ± 2.51%, 20.3 ± 3.21%, 28 ± 3.61 at 1,1-dichloroethane doses of 0, 100, 200, 300, 400 and 500 mg/kg BW respectively.


Cytogenetic effects of 1,1-dichloroethane in mice bone marrow cells.

Patlolla BP, Patlolla AK, Tchounwou PB - Int J Environ Res Public Health (2005)

Effect of 1,1-Dichloroethane on the frequency of chromosomal aberrations.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814703&req=5

f1-ijerph-02-00101: Effect of 1,1-Dichloroethane on the frequency of chromosomal aberrations.
Mentions: 1,1-Dichloroethane (DCE) induced a statistically significant increase in chromosome aberrations in metaphase bone marrow cells. Table. 1 shows the mean frequency of cells with aberrations (as percentages) calculated for each dose with 3 animals/dose. The most frequent types of aberrations were gaps, breaks and fragments. Chromatid-type aberrations were detected at high frequencies. Relatively higher frequencies of gaps were observed for all the doses tested. A quantitative assessment of the distribution of breaks and gaps revealed that the distal regions of the chromosomes were more vulnerable to the effects of 1,1-Dichloroethane. The frequency of chromosomal aberrations also increased with increasing doses of 1,1-dichloroethane (Figure 1). The mean percentages of the induced chromosomal aberrations were 2.67 ± 0.577 %, 7.66 ± 2.89 %, 8.33 ± 2.08 %, 14.67 ± 2.51%, 20.3 ± 3.21%, 28 ± 3.61 at 1,1-dichloroethane doses of 0, 100, 200, 300, 400 and 500 mg/kg BW respectively.

Bottom Line: Several chlorinated alkanes and alkenes were found to induce toxic effects.All animals were sacrificed 24 hours after the treatment.Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Alcorn State University, Lorman, MS, USA. babu@lorman.alcorn.edu

ABSTRACT
The major concern for the halogenated compounds is their widespread distribution, in addition to occupational exposures. Several chlorinated alkanes and alkenes were found to induce toxic effects. In this study, we investigated the genotoxic potential of 1,1-dichloroethane in the bone marrow cells obtained from Swiss-Webster mice, using chromosomal aberrations (CA), mitotic index (MI), and micronuclei (MN) formation as toxicological endpoints. Five groups of three male mice each, weighing an average of 24 +/- 2 g, were injected intraperitoneally, once with doses of 100, 200, 300, 400, 500 mg/kg body weight (BW) of 1,1-dichloroethane dissolved in ethanol. A control group was also made of three animals injected with ethanol (1%) without the chemical. All animals were sacrificed 24 hours after the treatment. Chromosome and micronuclei preparations were obtained from bone marrow cells following standard protocols. Chromatid and chromosome aberrations were investigated in 100 metaphase cells per animal and percent micronuclei frequencies were investigated in 1,000 metaphase cells per animal. 1,1-dichloroethane exposures significantly increased the number of chromosomal aberrations and the frequency of micronucleated cells in the bone marrow cells of Swiss-Webster mice. Percent chromosomal aberrations of 2.67 +/- 0.577, 7.66 +/- 2.89, 8.33 +/- 2.08, 14.67 +/- 2.51, 20.3 +/- 3.21, 28 +/- 3.61; mitotic index of 9.4%, 7.9%, 6.2%, 4.3%, 3.0%, 2.6% and micronuclei frequencies of 3.33 +/- 0.7, 7.33 +/- 0.9, 8.00 +/- 1.0, 11.67 +/- 1.2, 15.33 +/- 0.7, 18.00 +/- 1.7 were recorded for the control, 100, 200, 300, 400, and 500 mg/kg BW respectively; indicating a gradual increase in number of chromosomal aberrations and micronuclei formation, with increasing dose of 1,1,-dichloroethane. Our results indicate that 1,1-dichloroethane has a genotoxic potential as measured by the bone marrow CA and MN tests in Swiss-Webster mice.

Show MeSH
Related in: MedlinePlus