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Induced mitogenic activity in AML-12 mouse hepatocytes exposed to low-dose ultra-wideband electromagnetic radiation.

Dorsey WC, Ford BD, Roane L, Haynie DT, Tchounwou PB - Int J Environ Res Public Health (2005)

Bottom Line: Ultra-wideband (UWB) technology has increased with the use of various civilian and military applications.Cells were exposed to UWBR for 2 h at a temperature of 23 degrees C, a pulse width of 10 ns, a repetition rate of 1 kHz, and field strength of 5-20 kV/m.UWBR exerted a statistically significant (p < 0.05) dose-dependent response in cell viability in both serum-treated and serum free medium (SFM) -treated hepatocytes.

View Article: PubMed Central - PubMed

Affiliation: Wildlife Biology Unit, Grambling State University, Grambling, LA, USA.

ABSTRACT
Ultra-wideband (UWB) technology has increased with the use of various civilian and military applications. In the present study, we hypothesized that low-dose UWB electromagnetic radiation (UWBR) could elicit a mitogenic effect in AML-12 mouse hepatocytes, in vitro. To test this hypothesis, we exposed AML-12 mouse hepatocytes, to UWBR in a specially constructed gigahertz transverse electromagnetic mode (GTEM) cell. Cells were exposed to UWBR for 2 h at a temperature of 23 degrees C, a pulse width of 10 ns, a repetition rate of 1 kHz, and field strength of 5-20 kV/m. UWB pulses were triggered by an external pulse generator for UWBR exposure but were not triggered for the sham exposure. We performed an MTT Assay to assess cell viability for UWBR-treated and sham-exposed hepatocytes. Data from viability studies indicated a time-related increase in hepatocytes at time intervals from 8-24 h post exposure. UWBR exerted a statistically significant (p < 0.05) dose-dependent response in cell viability in both serum-treated and serum free medium (SFM) -treated hepatocytes. Western blot analysis of hepatocyte lysates demonstrated that cyclin A protein was induced in hepatocytes, suggesting that increased MTT activity after UWBR exposure was due to cell proliferation. This study indicates that UWBR has a mitogenic effect on AML-12 mouse hepatocytes and implicates a possible role for UWBR in hepatocarcinoma.

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Mitogenic effect of UWBR on HGM-treated AML-12 mouse hepatocytes. HGM-treated AML-12 mouse hepatocytes were exposed to UWB pulses for 2 h at a temp of 23°C, pulse width of 10 ns, repetition rate of 1 kHz, and applied field strength of 5–20 kV/m. Cell viability was assessed following a post-exposure incubation period of 8–24 h. In this assay, viable cells converted MTT to a water-insoluble formazan dye, as indicated in the methodology section. Bars are means ± SDs, n=3. *Significantly different from control, p ≤ 0.05.
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f2-ijerph-02-00024: Mitogenic effect of UWBR on HGM-treated AML-12 mouse hepatocytes. HGM-treated AML-12 mouse hepatocytes were exposed to UWB pulses for 2 h at a temp of 23°C, pulse width of 10 ns, repetition rate of 1 kHz, and applied field strength of 5–20 kV/m. Cell viability was assessed following a post-exposure incubation period of 8–24 h. In this assay, viable cells converted MTT to a water-insoluble formazan dye, as indicated in the methodology section. Bars are means ± SDs, n=3. *Significantly different from control, p ≤ 0.05.

Mentions: The effects of UWBR on the viability of AML-12 mouse hepatocytes are shown in Figure 2. In this experiment, we exposed hepatocytes to low-dose UWBR for 2 h, as indicated in the methodology section. Results from this experiment demonstrated a statistically significant (p<0.05) time-related increase in cell viability within the post-exposure periods of 8–24 h. The maximum increase in cell viability was 38% at the 24 h post-exposure period.


Induced mitogenic activity in AML-12 mouse hepatocytes exposed to low-dose ultra-wideband electromagnetic radiation.

Dorsey WC, Ford BD, Roane L, Haynie DT, Tchounwou PB - Int J Environ Res Public Health (2005)

Mitogenic effect of UWBR on HGM-treated AML-12 mouse hepatocytes. HGM-treated AML-12 mouse hepatocytes were exposed to UWB pulses for 2 h at a temp of 23°C, pulse width of 10 ns, repetition rate of 1 kHz, and applied field strength of 5–20 kV/m. Cell viability was assessed following a post-exposure incubation period of 8–24 h. In this assay, viable cells converted MTT to a water-insoluble formazan dye, as indicated in the methodology section. Bars are means ± SDs, n=3. *Significantly different from control, p ≤ 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814693&req=5

f2-ijerph-02-00024: Mitogenic effect of UWBR on HGM-treated AML-12 mouse hepatocytes. HGM-treated AML-12 mouse hepatocytes were exposed to UWB pulses for 2 h at a temp of 23°C, pulse width of 10 ns, repetition rate of 1 kHz, and applied field strength of 5–20 kV/m. Cell viability was assessed following a post-exposure incubation period of 8–24 h. In this assay, viable cells converted MTT to a water-insoluble formazan dye, as indicated in the methodology section. Bars are means ± SDs, n=3. *Significantly different from control, p ≤ 0.05.
Mentions: The effects of UWBR on the viability of AML-12 mouse hepatocytes are shown in Figure 2. In this experiment, we exposed hepatocytes to low-dose UWBR for 2 h, as indicated in the methodology section. Results from this experiment demonstrated a statistically significant (p<0.05) time-related increase in cell viability within the post-exposure periods of 8–24 h. The maximum increase in cell viability was 38% at the 24 h post-exposure period.

Bottom Line: Ultra-wideband (UWB) technology has increased with the use of various civilian and military applications.Cells were exposed to UWBR for 2 h at a temperature of 23 degrees C, a pulse width of 10 ns, a repetition rate of 1 kHz, and field strength of 5-20 kV/m.UWBR exerted a statistically significant (p < 0.05) dose-dependent response in cell viability in both serum-treated and serum free medium (SFM) -treated hepatocytes.

View Article: PubMed Central - PubMed

Affiliation: Wildlife Biology Unit, Grambling State University, Grambling, LA, USA.

ABSTRACT
Ultra-wideband (UWB) technology has increased with the use of various civilian and military applications. In the present study, we hypothesized that low-dose UWB electromagnetic radiation (UWBR) could elicit a mitogenic effect in AML-12 mouse hepatocytes, in vitro. To test this hypothesis, we exposed AML-12 mouse hepatocytes, to UWBR in a specially constructed gigahertz transverse electromagnetic mode (GTEM) cell. Cells were exposed to UWBR for 2 h at a temperature of 23 degrees C, a pulse width of 10 ns, a repetition rate of 1 kHz, and field strength of 5-20 kV/m. UWB pulses were triggered by an external pulse generator for UWBR exposure but were not triggered for the sham exposure. We performed an MTT Assay to assess cell viability for UWBR-treated and sham-exposed hepatocytes. Data from viability studies indicated a time-related increase in hepatocytes at time intervals from 8-24 h post exposure. UWBR exerted a statistically significant (p < 0.05) dose-dependent response in cell viability in both serum-treated and serum free medium (SFM) -treated hepatocytes. Western blot analysis of hepatocyte lysates demonstrated that cyclin A protein was induced in hepatocytes, suggesting that increased MTT activity after UWBR exposure was due to cell proliferation. This study indicates that UWBR has a mitogenic effect on AML-12 mouse hepatocytes and implicates a possible role for UWBR in hepatocarcinoma.

Show MeSH
Related in: MedlinePlus