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West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

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Number of WNV positive sera (red columns = both in ELISA and neutralization test (NT)) in horses from different Ukrainian regions. ELISA negative samples are depicted in green columns.
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viruses-05-02469-f003: Number of WNV positive sera (red columns = both in ELISA and neutralization test (NT)) in horses from different Ukrainian regions. ELISA negative samples are depicted in green columns.

Mentions: In summary, our results show that WNV circulates in large parts of Ukraine. In total, 42 horse sera (Table 3) tested positive for WNV antibodies as determined by both ELISA and NT. These samples were collected in seven regions, namely Kharkivska, Khmelnytska, Kyivska, Kirovohradska, Luhanska, Poltavska and Zaporizka (Figure 3, Table 4).


West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Number of WNV positive sera (red columns = both in ELISA and neutralization test (NT)) in horses from different Ukrainian regions. ELISA negative samples are depicted in green columns.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814598&req=5

viruses-05-02469-f003: Number of WNV positive sera (red columns = both in ELISA and neutralization test (NT)) in horses from different Ukrainian regions. ELISA negative samples are depicted in green columns.
Mentions: In summary, our results show that WNV circulates in large parts of Ukraine. In total, 42 horse sera (Table 3) tested positive for WNV antibodies as determined by both ELISA and NT. These samples were collected in seven regions, namely Kharkivska, Khmelnytska, Kyivska, Kirovohradska, Luhanska, Poltavska and Zaporizka (Figure 3, Table 4).

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

Show MeSH
Related in: MedlinePlus